Recombinant Mouse Anti-VACV A14 Antibody (GH3) (CAT#: FAMAB-0336CQ)

Figure 2 Categorization of the hybridomas into 11 groups according to the apparent molecular weight of the precipitated protein

HeLa cells were infected with VACV WR at a MOI of 10 and metabolically labeled with 35S-methionine and -cysteine from 8 to 16 hpi. The cells were lyzed and immunoprecipitated with the hybridoma supernatants and Protein G sepharose. The precipitated proteins were analyzed by SDS-PAGE, and the autoradiographs of representative samples are shown. The results are grouped together according to the size of the precipitated proteins and are shown from the smallest to the largest precipitated proteins. Shown above each lane is the clone name of the hybrdioma whose supernatant was used for the immunoprecipiation. The control immunoprecipitation with DMEM-10 medium is indicated by "–". The group number of the hybridomas is indicated below the lanes.

Meng, X., Zhong, Y., Embry, A., Yan, B., Lu, S., Zhong, G., & Xiang, Y. (2011). Generation and characterization of a large panel of murine monoclonal antibodies against vaccinia virus. Virology, 409(2), 271-279.