Mouse Anti-VWF Recombinant Antibody (clone SZ123)
CAT#: HPAB-M0250-YC
Provided is a human-mouse chimeric monoclonal antibody for a human von willebrand factor A3 region.
Gene Expression
Published Data
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF
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Figure 1 Anti-human VWF antibody SZ-123 cross-reacts with Rhesus monkey VWF.
SZ-123 blocked binding of plasma VWF from Rhesus monkey to human collagen type III coated plates and this binding is dose dependent.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
Figure 2 SZ-123 inhibits platelet thrombosis in vivo.
(A) Representative charts of CFRs showing the effect of SZ-123 on platelet thrombosis in femoral arteries of Rhesus monkeys. (B) Inhibition of CFRs measured within 1 h after administration of different doses of SZ-123.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
ELISA
Figure 3 Ex vivo analysis of plasma SZ-123 levels, VWF occupancy, and inhibition by SZ-123 on VWF binding to collagen.
(A) Plasma SZ-123 levels after administration of SZ-123. (B) VWF occupancy after administration of SZ-123. (C) Ex vivo inhibition by SZ-123 on Rhesus monkey VWF binding to collagen. (D) Correlation between ex vivo VWF occupancy and inhibition of VWF binding to collagen.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
Figure 4 SZ-123 inhibits VWF-dependent platelet agglutination.
Ex vivo inhibition of SZ-123 on ristocetin-induced Rhesus monkey platelet agglutination. Platelet agglutination was measured immediately after addition of 2.5 mg/mL ristocetin to PRP samples at each time point as indicated.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
Figure 5 SZ-123 does not affect VWF levels, platelet count, bleeding time, or coagulation
(A) No significant changes in platelet count were observed in animals after being treated with SZ-123 when compared with the t=0 injection of SZ-123. (B) Plasma VWF antigen levels were not affected by any treatment and no significant changes observed in after treated versus before SZ-123 injection.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
Figure 6 SZ-123 enhances VWF A3 binding to VWF A1 and inhibits the interaction between VWF A1 and GPIba.
(A) Radioimmunoassays measuring 125I-rVWF A3 binding to rVWF A1 coated on microtiter plates. (B) 125I-rVWF A3 binding to microtiter plates coated with rVWF A1 Binding assays were performed in the presence of different anti-VWF mAbs. (C) 125I-rVWF A1 was able to bind dose-dependently to rGPIba (H1-V289) induced by ristocetin. (D) SZ-123 but not 82D6A3 or SZ-34 inhibited 125I-rVWF A1 binding to rGPIba.
Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human VWF
- Species Reactivity
- Human, Monkey
- Clone
- SZ123
- Applications
- Inhib, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Von Willebrand Factor; F8VWF; Coagulation Factor VIII VWF; VWD; VWF;
- Gene ID
- 7450
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Reliable Inhibition of VWF Functions
Using this antibody has yielded consistent inhibition of VWF-collagen and platelet interactions. This has greatly advanced our understanding of thrombotic processes.
Excellent Support from Creative Biolabs
The technical support provided by Creative Biolabs has been exemplary. Their assistance in troubleshooting and optimizing assay conditions has enabled us to achieve precise results, enhancing the overall efficiency of our research endeavors.
Q&As
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What is the primary function of the Mouse Anti-VWF Recombinant Antibody (clone SZ123)?
A: The Mouse Anti-VWF Recombinant Antibody (clone SZ123) is designed to target the von Willebrand factor (VWF), primarily for studying the interactions of VWF in coagulation processes. It is used in various applications such as inhibition assays and functional studies to explore VWF's role in thrombus formation and hemostasis.
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Can this antibody be used in various species for comparative studies?
A: Yes, the Mouse Anti-VWF Recombinant Antibody reacts with human and Rhesus monkey VWF. This cross-reactivity facilitates comparative studies in different species, enhancing research into VWF-related vascular diseases.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone SZ123"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) | WB, IF, FuncS | Mouse IgG |
| PABW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4) | Block, Inhib, WB, DB | Mouse IgG |
| PABC-133 | Mouse Anti-vWF Recombinant Antibody (clone RU5) | ELISA, WB, IHC, IF, Inhib, FuncS | Mouse IgG |
| MOB-2032CT | Recombinant Mouse anti-Human VWF Monoclonal antibody (47C22) | IHC-Fr, IHC-P | |
| HPAB-0343-WJ | Mouse Anti-VWF Recombinant Antibody (HPAB-0343-WJ) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| PFBC-133 | Mouse Anti-VWF Recombinant Antibody (clone RU5); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
| HPAB-M0250-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); Fab Fragment | Inhib, FuncS | Mouse Fab |
| HPAB-M0251-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); Fab Fragment | Inhib, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); scFv Fragment | Block | Mouse scFv |
| PSBC-133 | Mouse Anti-VWF Recombinant Antibody (clone RU5); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
| FAMAB-0297-YC-S(P) | Human Anti-VWF Recombinant Antibody (clone h82D6A3); scFv Fragment | ELISA, WB | Humanized scFv |
| FAMAB-0298-YC-S(P) | Mouse Anti-VWF Recombinant Antibody (clone m-82D6A3); scFv Fragment | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362CQ | Anti-Human VWF Recombinant Antibody (AJvW-2) | ELISA, BL | |
| TAB-362CQ-S(P) | Anti-Human VWF Recombinant Antibody scFv Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-363CQ-S(P)) | ELISA, WB, Inhib | Mouse scFv |
| TAB-362CQ-F(E) | Anti-Human VWF Recombinant Antibody Fab Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (TAB-363CQ-F(E)) | ELISA, WB, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-364CQ | Human Anti-VWF Recombinant Antibody (TAB-364CQ) | Inhib | Humanized antibody |
| TAB-364CQ-S(P) | Human Anti-VWF Recombinant Antibody; scFv Fragment (TAB-364CQ-S(P)) | Inhib | Humanized scFv |
| TAB-364CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-364CQ-F(E)) | Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-365CQ | Human Anti-VWF Recombinant Antibody (TAB-365CQ) | Inhib | Chimeric antibody (mouse/human) |
| TAB-365CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-365CQ-S(P)) | Inhib | Mouse scFv |
| TAB-365CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-365CQ-F(E)) | Inhib | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3860 | Rabbit Anti-VWF Recombinant Antibody (clone DS3860AB) | WB, ICC, IF | Rabbit IgG |
| MOR-0109-FY | Rabbit Anti-VWF Recombinant Antibody (clone AFY0080) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1628-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1628-FY) | ELISA | Llama VHH |
| HPAB-1629-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1629-FY) | ELISA | Llama VHH |
| HPAB-1630-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1630-FY) | Inhib, ELISA | Llama VHH |
| HPAB-1631-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1631-FY) | ELISA, SPR, FC | Llama VHH |
| HPAB-1632-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1632-FY) | Inhib | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY41 | Human Anti-vWF (clone AJvW-2) scFv-Fc Chimera | ELISA, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2481 | Anti-VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7888 | Anti-Mouse VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7887 | Anti-Human VWF Immunohistochemistry Kit | IHC |
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