What is the primary function of the Mouse Anti-VWF Recombinant Antibody (clone SZ123)?

The Mouse Anti-VWF Recombinant Antibody (clone SZ123) is designed to target the von Willebrand factor (VWF), primarily for studying the interactions of VWF in coagulation processes. It is used in various applications such as inhibition assays and functional studies to explore VWF's role in thrombus formation and hemostasis.

Can this antibody be used in various species for comparative studies?

Yes, the Mouse Anti-VWF Recombinant Antibody reacts with human and Rhesus monkey VWF. This cross-reactivity facilitates comparative studies in different species, enhancing research into VWF-related vascular diseases.

Mouse Anti-VWF Recombinant Antibody (clone SZ123)

CAT#: HPAB-M0250-YC

Provided is a human-mouse chimeric monoclonal antibody for a human von willebrand factor A3 region.

Published Data Gene Expression

Figure 1 Anti-human VWF antibody SZ-123 cross-reacts with Rhesus monkey VWF.

SZ-123 blocked binding of plasma VWF from Rhesus monkey to human collagen type III coated plates and this binding is dose dependent.

Zhao, Y. M., Jiang, M., Ji, S. D., He, Y., Shen, F., Li, X. M., & Ruan, C. G. (2013). Anti-human VWF monoclonal antibody SZ-123 prevents arterial thrombus formation by inhibiting VWF-collagen and VWF-platelet interactions in Rhesus monkeys. Biochemical Pharmacology, 85(7), 945-953.

Figure 2 SZ-123 inhibits platelet thrombosis in vivo.

(A) Representative charts of CFRs showing the effect of SZ-123 on platelet thrombosis in femoral arteries of Rhesus monkeys. (B) Inhibition of CFRs measured within 1 h after administration of different doses of SZ-123.

ELISA

Figure 3 Ex vivo analysis of plasma SZ-123 levels, VWF occupancy, and inhibition by SZ-123 on VWF binding to collagen.

(A) Plasma SZ-123 levels after administration of SZ-123. (B) VWF occupancy after administration of SZ-123. (C) Ex vivo inhibition by SZ-123 on Rhesus monkey VWF binding to collagen. (D) Correlation between ex vivo VWF occupancy and inhibition of VWF binding to collagen.

Figure 4 SZ-123 inhibits VWF-dependent platelet agglutination.

Ex vivo inhibition of SZ-123 on ristocetin-induced Rhesus monkey platelet agglutination. Platelet agglutination was measured immediately after addition of 2.5 mg/mL ristocetin to PRP samples at each time point as indicated.

Figure 5 SZ-123 does not affect VWF levels, platelet count, bleeding time, or coagulation

(A) No significant changes in platelet count were observed in animals after being treated with SZ-123 when compared with the t=0 injection of SZ-123. (B) Plasma VWF antigen levels were not affected by any treatment and no significant changes observed in after treated versus before SZ-123 injection.

Figure 6 SZ-123 enhances VWF A3 binding to VWF A1 and inhibits the interaction between VWF A1 and GPIba.

(A) Radioimmunoassays measuring ¹²⁵I-rVWF A3 binding to rVWF A1 coated on microtiter plates. (B) ¹²⁵I-rVWF A3 binding to microtiter plates coated with rVWF A1 Binding assays were performed in the presence of different anti-VWF mAbs. (C) ¹²⁵I-rVWF A1 was able to bind dose-dependently to rGPIba (H1-V289) induced by ristocetin. (D) SZ-123 but not 82D6A3 or SZ-34 inhibited ¹²⁵I-rVWF A1 binding to rGPIba.

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RNA Expression Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF

Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)

Cell lines ordered by descending RNA expression order.

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human VWF

Species Reactivity
Human, Monkey

Clone
SZ123

Applications
Inhib, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Von Willebrand Factor; F8VWF; Coagulation Factor VIII VWF; VWD; VWF;

Gene ID
7450

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Reliable Inhibition of VWF Functions

Using this antibody has yielded consistent inhibition of VWF-collagen and platelet interactions. This has greatly advanced our understanding of thrombotic processes.

Excellent Support from Creative Biolabs

The technical support provided by Creative Biolabs has been exemplary. Their assistance in troubleshooting and optimizing assay conditions has enabled us to achieve precise results, enhancing the overall efficiency of our research endeavors.

Q&As

What is the primary function of the Mouse Anti-VWF Recombinant Antibody (clone SZ123)?

A: The Mouse Anti-VWF Recombinant Antibody (clone SZ123) is designed to target the von Willebrand factor (VWF), primarily for studying the interactions of VWF in coagulation processes. It is used in various applications such as inhibition assays and functional studies to explore VWF's role in thrombus formation and hemostasis.
Can this antibody be used in various species for comparative studies?

A: Yes, the Mouse Anti-VWF Recombinant Antibody reacts with human and Rhesus monkey VWF. This cross-reactivity facilitates comparative studies in different species, enhancing research into VWF-related vascular diseases.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-M0250-YC, RRID: AB_3713881)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-M0250-YC.pdf

MSDS

MSDS - HPAB-M0250-YC.pdf

COA

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