Creative Biolabs is specialized in providing bispecific antibody analysis services, and now offers customers elaborate analysis strategy for completely assessing your bispecific antibody (BsAb) stability. Our experts have rich experience in BsAb stability analysis. Based on our advanced instrument and extensive expertise, we can provide clients with various stability analytical approaches to meet your requirements exactly.
Our stability analysis services for bispecific antibody include:
Thermal stability assessment
Creative biolabs offers two methods, DSC and DSF, for the analysis of the melting temperature (T (m)) of your BsAbs. DSC refers to Differential Scanning Calorimetry, which measures phase transitions of a molecule when it is being heated, and provides an assessment of thermal stability; while DSF, known as Differential Scanning Fluorimetry, is a high-throughput screening method for analysis of protein T (m). As a commonly used polarity-sensitive dye for DSF measurement, SYPRO orange is a hydrophobic fluorescent and shows bright fluorescence in the presence of micelles, concealing the signal of protein unfolding. The molecular rotor probe 4-(dicyanovinyl) julolidine (DCVJ) is also available upon request.
Aggregation temperature and rate measurement by Dynamic Light Scattering (DLS)
Dynamic Light Scattering (DLS), also called Photon Correlation Spectroscopy, is a sensitive method for detecting macromolecule aggregation in solution which can accurately measure the fluctuations of light scattering intensity that are resulted from molecules diffusion in a very short time scale. When analyzing with DLS, the temperature at which particle size begins to increase is defined as the aggregation temperature. And aggregation strengthens from this certain temperature when samples are being heated up gradually. The particle size increases and further changes the diffusion of lights. Hence, the aggregation tendency of a molecule can be monitored over time by continuous acquisition of DLS data. However, other analysis approaches are also available upon request.
The measurement of in vitro serum stability
The stability in human serum is an important factor affecting the therapeutic efficacy of antibodies, so it is quite meaningful to analyze the in vitro serum stability of BsAbs. ELISA and Flow-cytometry are both available for determining the concentration of active BsAb molecule after being incubated with human serum at 37 °C for a certain time. Half-life is calculated as the final data to evaluate the in vitro stability of your BsAbs.