Recombinant Mouse Anti-ACP3 Antibody (clone 6E2-2D2-5F4) (CAT#: VS3-FY19)

• Immunogen
• Synthesized peptide derived from human Prostatic Acid Phosphatase(PSAP)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human ACP3

• Species Reactivity
• Human

• Clone
• 6E2-2D2-5F4

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• PBS pH 7.3, 50% Glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry: 1/100-1/500

• Alternative Names
• 5' -NT; 5' -nucleotidase; ACP3; PAP; Prostatic acid phosphatase; TMPase

• Gene ID
• 55

• UniProt ID
• P15309

• Sequence Similarities
• Belongs to the histidine acid phosphatase family.

• Cellular Localization
• Amyloid, Cell membrane, Cytoplasm, Lysosome, Membrane, Nucleus, Secreted

• Post Translation Modifications
• N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
  Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection).

• Protein Refseq
• NP_001090.2; NP_001127666.1; NP_001278966.1

• Function
• A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846).
  Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042).
  Isoform 2:
  Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed:20498373).
  In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).
  PAPf39:
  (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482).
  SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623).
  Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed:19897482).
  Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677).

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS