Recombinant Rabbit Anti-PTGS1 Antibody (clone R02-6G4)
CAT#: VS3-FY1214
This product is a recombinant rabbit antibody that recognizes PTGS1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-6G4 is specific for human, mouse, rat PTGS1. The antigen is synthetic peptide of human cox1/cyclooxygenase 1.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2834/1446_E11_4_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, HEL, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095303-PTGS1/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-PTGS1 Antibody (clone R02-6G4) in WB.
Western Blot analysis of COX1/Cyclooxygenase 1 in C6, 3T3 lysates using a COX1/Cyclooxygenase 1 antibody.
ICC
Figure 2. Recombinant Rabbit Anti-PTGS1 Antibody (clone R02-6G4) in ICC.
Immunocytochemical analysis of COX1/Cyclooxygenase 1 (green) in HeLa using a COX1/Cyclooxygenase 1 antibody and DAPI (blue).
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Specifications
- Immunogen
- Synthetic peptide of human COX1/cyclooxygenase 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PTGS1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6G4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 69 kDa; Observed MW: 69 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PTGS1; COX1; Prostaglandin G/H synthase 1; Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1
- Gene ID
- 5742
- UniProt ID
- P23219
- Sequence Similarities
- Belongs to the prostaglandin G/H synthase family.
- Cellular Localization
- Endoplasmic reticulum, Membrane, Microsome
- Protein Refseq
- NP_000953.2; NP_001258094.1; NP_001258095.1
- Function
- Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975).
Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTGS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1999z | Mouse Anti-PTGS1 Recombinant Antibody (MOB-1999z) | WB, IP, IF, IHC-P, FC | Mouse IgG2b, κ |
| MOB-0714MZ | Recombinant Mouse Anti-Human PTGS1 Antibody (clone BT80) | ELISA | Mouse antibody |
| MRO-1787-CN | Rabbit Anti-PTGS1 Polyclonal Antibody (MRO-1787-CN) | WB, IF | Rabbit IgG |
| VS3-CJ790 | Rabbit Anti-PTGS1 Recombinant Antibody (VS3-CJ790) | WB, ICC, IHC, FC, IP | Rabbit IgG |
| VS-0224-XY103 | Mouse Anti-PTGS1 Recombinant Antibody (VS-0224-XY103) | ELISA, WB, IF, IHC, ICC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1999z-F(E) | Recombinant Anti-Human PTGS1 Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1999z-S(P) | Recombinant Anti-Human PTGS1 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2921 | Hi-Affi™ Recombinant Rabbit Anti-PTGS1 Monoclonal Antibody (DS2921AB) | WB, IP, IHC-P, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5835 | Anti-PTGS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5836 | Anti-Mouse PTGS1 Immunohistochemistry Kit | IHC |
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