Recombinant Rabbit Anti-TNF Capture Antibody (clone R02-4W7)
CAT#: VS3-WK1790
This antibody is a rabbit recombinant antibody against rat TNF. It was developed by immunization of rabbit with recombinant rat TNF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R02-4W7 and R01-4I9. The clone R02-4W7 can be used as a capture antibody in ELISA analysis to identify rat TNF.
Gene Expression
Normal Tissue
Figure 1 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF
❮
❯
Specifications
- Immunogen
- Recombinant rat TNF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Rat TNF
- Species Reactivity
- Rat
- Clone
- R02-4W7
- Applications
- ELISA
- Conjugate
- Unconjugated
- Related Disease
- Rheumatoid arthritis and psoriasis
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Tnfa; RATTNF; TNF-alpha
- Gene ID
- 24835
- UniProt ID
- P16599
- Long Name
- Tumor necrosis factor
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.
- Protein Refseq
- NP_000585.2
- Function
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
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Related Diseases
Asthma
Inflammatory Bowel Diseases
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
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