Recombinant Rabbit Anti-TNF Detection Antibody (clone R01-4I9)

Name: Recombinant Rabbit Anti-TNF Detection Antibody (clone R01-4I9)
SKU: VS3-WK1791
Rating: 5 (1 reviews)

CAT#: VS3-WK1791

This antibody is a rabbit recombinant antibody against rat TNF. It was developed by immunization of rabbit with recombinant rat TNF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R02-4W7 and R01-4I9 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R01-4I9 can be used in ELISA analysis to identify rat TNF.

Gene Expression

Normal Tissue Figure 1 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF

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Specifications

Immunogen
Recombinant rat TNF protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Rat TNF

Species Reactivity
Rat

Clone
R01-4I9

Applications
ELISA

Conjugate
Unconjugated

Related Disease
Rheumatoid arthritis and psoriasis

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Aggregation
< 5%

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
0.01M PBS, pH 7.2

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
Tnfa; RATTNF; TNF-alpha

Gene ID
24835

UniProt ID
P16599

Long Name
Tumor necrosis factor

Sequence Similarities
Belongs to the tumor necrosis factor family.

Cellular Localization
Cell membrane, Membrane, Secreted

Post Translation Modifications
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.

Protein Refseq
NP_000585.2

Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective.

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Related Diseases

Asthma

Inflammatory Bowel Diseases

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK1791.pdf

MSDS

MSDS - VS3-WK1791.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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