Recombinant Rabbit Anti-TNF Detection Antibody (clone R01-4I9) (CAT#: VS3-WK1791)

• Immunogen
• Recombinant rat TNF protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Rat TNF

• Species Reactivity
• Rat

• Clone
• R01-4I9

• Applications
• ELISA

• Conjugate
• Unconjugated

• Related Disease
• Rheumatoid arthritis and psoriasis

• Clonality
• Monoclonal

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 0.01M PBS, pH 7.2

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Alternative Names
• Tnfa; RATTNF; TNF-alpha

• Gene ID
• 24835

• UniProt ID
• P16599

• Long Name
• Tumor necrosis factor

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.

• Protein Refseq
• NP_000585.2

• Function
• Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective.

Capture antibody

• VS3-WK1790
• Recombinant Rabbit Anti-TNF Capture Antibody (clone R02-4W7)

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2RD-A1517-YJ
• Sheep Anti-Rabbit IgG-heavy and light chain Secondary antibody

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