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Oligonucleotide

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For Research Use Only. Not For Clinical Use.


Background

An oligonucleotide is a short sequence of nucleotides, typically less than 50 nucleotides in length, that is used in various applications in molecular biology, genetics, and biotechnology. Oligonucleotides can be synthesized chemically and are commonly used as primers in PCR (polymerase chain reaction), probes for detecting specific DNA sequences, and as tools for gene editing and manipulation.
These molecules are made up of individual units called nucleotides, which are composed of a sugar (deoxyribose in DNA or ribose in RNA), a phosphate group, and a nitrogenous base (adenine, guanine, cytosine, or thymine in DNA; or uracil in RNA). The specific sequence of nucleotides in an oligonucleotide determines its function and specificity for binding to complementary sequences in DNA or RNA.
Oligonucleotides play a crucial role in many research and diagnostic applications, such as gene expression analysis, genotyping, and antisense therapy. They are versatile tools that have revolutionized the field of molecular biology and continue to be widely used in various scientific disciplines.
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