Anti-A33 Recombinant Antibody (HmAb A33) (CAT#: TAB-0626CLV)

The anti-A33 antibody specifically bind to A33, and can be used in the treatment of cancers, such as colon cancer and stomach cancer.


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Figure 1 Surface Plasmon resonance. Association and dissociation curves of A33 antibody preparations on an A33 antigen-coated biochip.

Figure 1 Surface Plasmon resonance. Association and dissociation curves of A33 antibody preparations on an A33 antigen-coated biochip.

Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.

Cyt

Figure 2 A33scFv-CD-mediated cytotoxicity on A33 antigen-positive vs negative cells.

Figure 2 A33scFv-CD-mediated cytotoxicity on A33 antigen-positive vs negative cells.

LIM1215 cells and HT29 cells were incubated with a dilution series of A33scFv-CD fusion protein and, after washing, with the 5-FC prodrug. Survival was measured by the MTT method as described. A33scFv-CD fusion protein from two different preparations was used on HT29 cells (▴ and ▾) and on LIM1215 cells (• and◆). As a control, a single, high concentration of A33scFv-GFP (□) was used instead of A33scFv-CD. Mean and s.d. of triplicate samples.

Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.

BL

Figure 3 MTT cytotoxicity blocking assay.

Figure 3 MTT cytotoxicity blocking assay.

As a negative control, A33scFv-CD was used without subsequent prodrug incubation (▪), and 5-FU alone served as positive control (▴). In the complete ADEPT assay with subsequent 5-FC incubation as described in the text, cells were preincubated either with the "A33scFv-GFP" antibody (▫) or with hu3S193 as an isotypic control antibody (▾) for 1 h before the fusion protein was added. Mean and s.d. of triplicate samples.

Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.


Specifications

  • Host Species
  • Human
  • Clone
  • HmAb A33
  • Applications
  • Cyt, BL
  • Related Disease
  • Colon cancer, Stomach cancer

Applications

  • Application Notes
  • For cytotoxicity assays, LIM 1215 or HT 29 tumour cells were incubated on 96-well plates to reach 25-33% surface density. Fusion protein or control was added for 60 min, preceded by 90 min of incubation with "A33scFv-GFP" or hu3S193 IgG (1 mg/ml) in blocking experiments. After washing, cells were incubated with prodrug or control for 48 h, washed and grown in medium for 72 h, followed by 3 h in 0.5 mg ml−1 MTT-solution, DMSO-lysis and photometry at 595 nm.

Target

  • Alternative Names
  • A33; glycoprotein A33 (transmembrane); GPA3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Human Antibody

CAT Product Name Application Type
TAB-002CL Anti-Human A33 Recombinant Antibody (KRN330) IHC Antibody

Humanized Antibody

CAT Product Name Application Type
TAB-0620CLV Anti-Human A33 Recombinant Antibody (A) FC, IF Humanized antibody

Recombinant Antibody

CAT Product Name Application Type
HPAB-0127-FY Human Anti-A33 Recombinant Antibody (HPAB-0127-FY) ELISA, Neut, FuncS Human IgG

scFv Fragment Antibody

Fab Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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