Anti-A33 Recombinant Antibody (HmAb A33)
CAT#: TAB-0626CLV
The anti-A33 antibody specifically bind to A33, and can be used in the treatment of cancers, such as colon cancer and stomach cancer.
Published Data
Figure 1 Surface Plasmon resonance. Association and dissociation curves of A33 antibody preparations on an A33 antigen-coated biochip.
Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.
Cyt
Figure 2 A33scFv-CD-mediated cytotoxicity on A33 antigen-positive vs negative cells.
LIM1215 cells and HT29 cells were incubated with a dilution series of A33scFv-CD fusion protein and, after washing, with the 5-FC prodrug. Survival was measured by the MTT method as described. A33scFv-CD fusion protein from two different preparations was used on HT29 cells (▴ and ▾) and on LIM1215 cells (• and◆). As a control, a single, high concentration of A33scFv-GFP (□) was used instead of A33scFv-CD. Mean and s.d. of triplicate samples.
Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.
BL
Figure 3 MTT cytotoxicity blocking assay.
As a negative control, A33scFv-CD was used without subsequent prodrug incubation (▪), and 5-FU alone served as positive control (▴). In the complete ADEPT assay with subsequent 5-FC incubation as described in the text, cells were preincubated either with the "A33scFv-GFP" antibody (▫) or with hu3S193 as an isotypic control antibody (▾) for 1 h before the fusion protein was added. Mean and s.d. of triplicate samples.
Deckert, P. M., Renner, C., Cohen, L. S., Jungbluth, A., Ritter, G., Bertino, J. R.,... & Welt, S. (2003). A33scFv–cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British journal of cancer, 88(6), 937.
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Specifications
- Host Species
- Human
- Clone
- HmAb A33
- Applications
- Cyt, BL
- Related Disease
- Colon cancer, Stomach cancer
Applications
- Application Notes
- For cytotoxicity assays, LIM 1215 or HT 29 tumour cells were incubated on 96-well plates to reach 25-33% surface density. Fusion protein or control was added for 60 min, preceded by 90 min of incubation with "A33scFv-GFP" or hu3S193 IgG (1 mg/ml) in blocking experiments. After washing, cells were incubated with prodrug or control for 48 h, washed and grown in medium for 72 h, followed by 3 h in 0.5 mg ml−1 MTT-solution, DMSO-lysis and photometry at 595 nm.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002CL | Anti-Human A33 Recombinant Antibody (KRN330) | IHC | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0620CLV | Anti-Human A33 Recombinant Antibody (A) | FC, IF | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0629CLV | Mouse Anti-A33 Recombinant Antibody (TAB-0629CLV) | ELISA, IHC | Mouse IgG |
| TAB-0629CLV-F(E) | Mouse Anti-A33 Recombinant Antibody; Fab Fragment (TAB-0629CLV-F(E)) | ELISA, IHC | Mouse Fab |
| TAB-0629CLV-S(P) | Mouse Anti-A33 Recombinant Antibody; scFv Fragment (TAB-0629CLV-S(P)) | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-FY | Human Anti-A33 Recombinant Antibody (HPAB-0127-FY) | ELISA, Neut, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-FY-S(P) | Human Anti-A33 Recombinant Antibody; scFv Fragment (HPAB-0127-FY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-FY-F(E) | Human Anti-A33 Recombinant Antibody; Fab Fragment (HPAB-0127-FY-F(E)) | ELISA | Human Fab |
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