Recombinant Anti-human ADAM10 Intrabody [(D-Arg)9]
CAT#: IAB-B003(A)
Recombinant anti-ADAM10 intrabody linked to (D-Arg)9 works within cells to bind selectively to intracellur antigen human ADAM10, expressed in E. coli cells. The anti-ADAM10 intrabodies are designed from either the polyclonal or monoclonal anti-ADAM10 antibody cDNA that encode antibodiess that recognize the enzymatically active form of Kuz and which, upon binding, inhibit ADAM10's ability to transphosphorylate.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50670/757_F11_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/211_C7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/4396_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/4396_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/4396_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/4396_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1709/4396_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137845-ADAM10
❮
❯
Specifications
- Immunogen
- human ADAM metallopeptidase domain 10
- Host Species
- human
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Products can be used for: Protein fragment complementation; Western blot; Functional studies
Product Property
- Purity
- >95%, by SDS-PAGE, under reducing conditions
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Target
- Alternative Names
- ADAM10; ADAM metallopeptidase domain 10; RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717; disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprote
- Gene ID
- 102
- UniProt ID
- A0A024R5U5
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for IAB-B003(A). Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ADAM10"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2188z | Mouse Anti-ADAM10 Recombinant Antibody (clone 8A6) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-0029-CN | Rabbit Anti-ADAM10 Recombinant Antibody (clone JM32-11) | WB, IP | Rabbit IgG |
| MRO-1627-CN | Rabbit Anti-ADAM10 Polyclonal Antibody (MRO-1627-CN) | WB, IHC | Rabbit IgG |
| VS3-FY2620 | Mouse Anti-ADAM10 Recombinant Antibody (VS3-FY2620) | IHC, FC, ELISA | Mouse IgG |
| VS7-HM38 | Mouse Anti-ADAM10 Recombinant Antibody (clone 6C3C7) | IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B003(G) | Recombinant Anti-human ADAM10 Intrabody [+36 GFP] | FACS, ELISA, IF, FuncS | scFv-(+36GFP) |
| IAB-B003(T) | Recombinant Anti-human ADAM10 Intrabody [Tat] | FACS, ICC, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0068 | Hi-Affi™ Rabbit Anti-ADAM10 Recombinant Antibody (clone DS68AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-YJ-S(P) | Mouse Anti-ADAM10 Recombinant Antibody; scFv Fragment (HPAB-0101-YJ-S(P)) | IP, Inhib, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-YJ-F(E) | Mouse Anti-ADAM10 Recombinant Antibody; Fab Fragment (HPAB-0101-YJ-F(E)) | IP, Inhib, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY40 | Anti-ADAM10 Immunohistochemistry Kit | IHC | |
| VS-0525-XY146 | Anti-Mouse ADAM10 Immunohistochemistry Kit | IHC | |
| VS-0525-XY145 | Anti-Human ADAM10 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC400 | Recombinant Anti-ADAM10 Vesicular Antibody, EV Displayed (VS-0425-YC400) | ELISA, FC, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, IF, FuncS
Application: WB, IF, FuncS
Application: Neut, FuncS, IHC, ELISA, FC, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
