Recombinant monoclonal antibody to CLDN1. 3A2 is a mouse monoclonal antibody that can be potentially used in the treatment of hepatitis C.
Figure 1 Establishment of mouse anti-hCLDN1 MAbs.
Huh7.5.1-8 (black histograms) or S7-A (white histograms) cells were incubated with the conditioned medium from each hybridoma clone and treated with phycoerythrin-conjugated secondary Abs. Stained cells were analyzed by flow cytometry.
Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.
Figure 2 Measurement of anti-CLDN1 MAb binding to cellular CLDN1 by cell ELISA.
After fixation, Huh7.5.1-8 cells in 96-well plates were incubated with serial dilutions of each anti-CLDN1 MAb (2C1, 3A2, 5F2, and 7A5) or control IgG, followed by horseradish peroxidase-conjugated second antibody.
Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.
Figure 3 Cell lysates were subjected to immunoblotting of HCV NS3, core, and GAPDH proteins.
Huh7.5.1-8 cells were seeded at 5×10^4 cells in 48-well plates and pretreated with the indicated amount of anti-hCLDN1 MAb or control IgG (control Ab) for 1 h at room temperature and then were subjected to the following procedures. Cells were infected with HCVcc (MOI = 1) for 2 h at room temperature and cultured for 4 days in the presence of the indicated amounts (0.1 to 5 μg/well) of MAbs (400 μl/well).
Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.
Figure 4 Inhibition of HCV infection by anti-hCLDN1 MAbs in vitro.
Cells were then infected with HCVpp (genotype 2a) for 3 h atroom temperature and cultured for 2 days in the presence of the indicated amounts of each anti-hCLDN1 MAb. Luciferase activities in cell lysates were measured using a luminometer. Values are expressed as percentages. Data in each graph represent the means ±SD (n=3). RLU, relative light units.
Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.
Figure 5 Anti-CLDN1 MAbs did not affect TJ functions in cultured cells.
Huh7.5.1-8 cells were treated with or without 5 μg of anti-hCLDN1 MAbs and cultured for 4 days at 37°C. Cells were fixed, permeabilized, and stained with anti-CLDN1 pAbs, which recognize the cytosolic domain of CLDN1.
Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-594-VHH | Recombinant Anti-Human CLDN1 VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-479LC-S(P) | Anti-Human CLDN1 Recombinant Antibody scFv Fragment (3A2) | FC, IF | |
TAB-480LC-S(P) | Anti-Human CLDN1 Recombinant Antibody scFv Fragment (2C1) | FC, IF | |
TAB-481LC-S(P) | Anti-Human CLDN1 Recombinant Antibody scFv Fragment (5F2) | FC, IF | |
TAB-482LC-S(P) | Anti-Human CLDN1 Recombinant Antibody scFv Fragment (7A5) | FC, IF | |
TAB-483LC-S(P) | Mouse Anti-CLDN1 Recombinant Antibody; scFv Fragment (TAB-483LC-S(P)) | WB, FC, IP | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1101MZ | Recombinant Mouse Anti-Human CLDN1 Antibody | IHC-P | Mouse antibody |
HPAB-M0488-YC | Rat Anti-CLDN1 Recombinant Antibody (HPAB-M0488-YC) | FC, Inhib, FuncS | Rat IgG2b, κ |
HPAB-M0489-YC | Human Anti-CLDN1 Recombinant Antibody (HPAB-M0489-YC) | FC, Inhib, FuncS | Humanized IgG |
ZG-0154J | Mouse Anti-CLDN1 Recombinant Antibody (ZG-0154J) | IHC-P, IF, WB | Mouse IgG |
VS3-CJ195 | Mouse Anti-CLDN1 Recombinant Antibody (clone PT0509) | IHC-P, WB |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0716 | Hi-Affi™ Rabbit Anti-CLDN1 Recombinant Antibody (clone DS716AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0080-LS-S(P) | Mouse Anti-CLDN1 Recombinant Antibody; scFv Fragment (HPAB-0080-LS-S(P)) | WB, FC, IF, IP, Inhib | Mouse scFv |
HPAB-M0488-YC-S(P) | Rat Anti-CLDN1 Recombinant Antibody; scFv Fragment (HPAB-M0488-YC-S(P)) | FC, Inhib, FuncS | Rat scFv |
HPAB-M0489-YC-S(P) | Human Anti-CLDN1 Recombinant Antibody; scFv Fragment (HPAB-M0489-YC-S(P)) | FC, Inhib, FuncS | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0080-LS-F(E) | Mouse Anti-CLDN1 Recombinant Antibody; Fab Fragment (HPAB-0080-LS-F(E)) | WB, FC, IF, IP, Inhib | Mouse Fab |
HPAB-M0488-YC-F(E) | Rat Anti-CLDN1 Recombinant Antibody; Fab Fragment (HPAB-M0488-YC-F(E)) | FC, Inhib, FuncS | Rat Fab |
HPAB-M0489-YC-F(E) | Human Anti-CLDN1 Recombinant Antibody; Fab Fragment (HPAB-M0489-YC-F(E)) | FC, Inhib, FuncS | Humanized Fab |
There are currently no Customer reviews or questions for TAB-479LC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.