Anti-EDB Antibody-Cytokine Fusion Protein, IgG-IL12 (ACFP-SH087) (CAT#: ACFP-SH087)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EDB IgG to IL12. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EDB as well as the biological activity of IL12. It was found that huBC1-huIL12 has a longer serum half-life than antibody-muIL12 in mice. The antibody-huIL12 is a molecule designed to target tumor vasculature and evoke a localized immune cascade. The antibody-(IL12) demonstrated 10-fold lower IL12 activity compared with rmIL12 as determined by the level of IFN-γ secreted by PBMC. This immunocytokine was designed for treating Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
Figure 2 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
Figure 3 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
Figure 4 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
Figure 5 Placenta
(Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
(Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
Figure 6 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
Specifications
- Target
- EDB
- Molecule Class
- IgG1-IL12
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- HuBC1
- Related Disease
- Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma
- Cytokine 1
- IL12
- Cytokines 1 Description
- Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
Related Resources
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone HuBC1"
See other products for "FN1"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
Single Domain Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-071WM-S(P) | Anti-Human FN1 Recombinant Antibody scFv Fragment (CGS-1) | ELISA, WB | Human Antibody |
| TAB-001CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (MOR03255) | ELISA, BL, IHC | Human antibody |
| TAB-002CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (ME-4C) | ELISA, IHC | Human antibody |
| TAB-003CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (1C5) | ELISA, WB | Human antibody |
| TAB-004CQ-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (TAB-004CQ-F(E)) | ELISA, IHC, WB | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ACFP-SH087. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
![Figure 1 Anti-Human CD19 Recombinant Antibody Fab Fragment [TAB-1611CL-F(E)] in SDS-PAGE](https://img.creativebiolabs.net/productimages/COA-TAB-1611CL-F(E)-1.png)
Application: Depletion, FuncS
Application: Neut, FC, IHC-Fr, IP, BA
Application: WB, ELISA, FC, IHC, IF, IP
Application: Neut, IHC, Activ, FuncS, IF, ELISA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
