Rabbit Anti-ERBB2 Recombinant Antibody (VS-1125-XY409)

Name: Rabbit Anti-ERBB2 Recombinant Antibody (VS-1125-XY409)
SKU: VS-1125-XY409
Rating: 5 (1 reviews)

CAT#: VS-1125-XY409

Suitable for integration into cell therapy protocols, this ERBB2 recombinant antibody helps regulate ERBB2-mediated signaling in engineered cells, and it also functions effectively in companion diagnostics to assess ERBB2 expression in patient samples. It undergoes rigorous quality control to meet the standards of both therapeutic and diagnostic use.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

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Specifications

Host Animal
Rabbit

Applications
IHC, ICC, IF, FC

Specificity
ERBB2

Target
ERBB2

Species Reactivity
Human

Type
Rabbit IgG, kappa

Purity
>95%, determined by SDS-PAGE

Purification
Protein A purified

Format
Liquid

Buffer
PBS, pH 7.4, with 0.05% BSA

Preservative
0.05% sodium azide

Storage
Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Target

Introduction
ERBB2 (human epidermal growth factor receptor 2, HER2) is a transmembrane tyrosine kinase receptor (ErbB family) with no known ligand, activating signaling via dimerization with other ErbB receptors. Amplification or overexpression drives tumorigenesis in breast cancer, gastric/GEJ cancer, ovarian cancer, and metastatic colorectal cancer. HER2 is a well-established therapeutic target: trastuzumab (monoclonal antibody), pertuzumab (bispecific antibody), and TKIs (lapatinib, tucatinib) block HER2 signaling, while ADCs (ado-trastuzumab emtansine) deliver cytotoxic agents to HER2+ cells. HER2 targeting has drastically improved outcomes for HER2-positive cancers, with combination therapies enhancing efficacy.

Alternative Names
CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2)

Gene ID
2064

UniProt ID
P04626

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-1125-XY409.pdf

MSDS

MSDS - VS-1125-XY409.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Secondary Antibody

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Product Name

Isotype Control

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