Recombinant Human Anti-H3N2 HA Antibody (C05) (CAT#: PABZ-198)

Recombinant Human Antibody (C05) is capable of binding to H3N2 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H3N2 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H3N2 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. C5 recognizes conserved elements of the receptor binding site on the hemagglutinin (HA) surface glycoprotein.

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Figure 1 Impact of L194P substitution on antigenicity.

Figure 1 Impact of L194P substitution on antigenicity.

(B-C) Biolayer interferometry (BLI) was used to measure the binding kinetics of C05 IgG against the recombinant HA proteins of (B) Bris07 P194 and (C) Bris07 L194. (D) HAI assay of C05 IgG against Bris07 P194 and Bris07 L194 viruses. Duplicates were performed for each of Bris07 P194 and L194.

Wu, N. C., Zost, S. J., Thompson, A. J., Oyen, D., Nycholat, C. M., McBride, R., ... & Wilson, I. A. (2017). A structural explanation for the low effectiveness of the seasonal influenza H3N2 vaccine. PLoS pathogens, 13(10), e1006682.

Figure 2 C05 protects mice from lethal virus challenge.

Figure 2 C05 protects mice from lethal virus challenge.

a, b, Survival and weight loss were monitored in response to varying amounts of C05 IgG administered prophylactically to mice 24 h before challenge with 25 × the 50% mouse lethal dose (MLD₅₀) of A/Memphis/3/2008 (H1N1) (a) or 333 MLD₅₀ of A/X-31/1968 (H3N2) viruses (b). c, d, A single therapeutic dose of 15 mg/kg C05 IgG was delivered 1, 2, 3, 4 or 5 days post-infection (DPI) with 25 × MLD₅₀ of A/Memphis/3/2008 (c) or 33 × MLD₅₀ of A/X-31/1968 viruses (d). n = 5 mice for all studies. Error bars denote ± s.d.

Ekiert, D. C., Kashyap, A. K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R., … Wilson, I. A. (2012). Cross-neutralization of influenza A viruses mediated by a single antibody loop. Nature, 489(7417), 526–532.


  • Immunogen
  • IAV Hemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H3N2 HA
  • Species Reactivity
  • IAV
  • Clone
  • C05
  • Applications
  • Neut Assay-Dependent
    Hemagglutination inhibition assay 48 pg/mL-200 μg/mL
    FuncS Assay-Dependent

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.


  • Application Notes
  • The antibody C05 has been reported in applications of Neut, Hemagglutination inhibition assay, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
    Hemagglutination inhibition assay: A reported concentration of the antibody C05 for hemagglutination inhibition assay was 48 pg/mL-200 μg/mL.


  • Alternative Names
  • HA; Hemagglutinin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.


Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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