What is the primary application for this HER2 antibody?

This Anti-HER2/neu Recombinant Antibody is validated for Western Blot (WB) applications. It is designed to detect Human HER2 (ERBB2) protein in various samples, such as cell lysates from breast cancer lines.

Is this a human or mouse antibody?

This is a Recombinant Human antibody. It is derived from a human antibody phage display library and expressed in CHO cells, making it a fully human IgG molecule.

Anti-Human HER2/neu Recombinant Antibody

CAT#: TAB-055CT

Recombinant monoclonal antibody to HER2/neu. This antibody intended for the prophylaxis and treatment of HER2-positive breast cancer.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

❮

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Specifications

Immunogen
Recombinant Human ErbB2/Her2 Fc Chimera Protein

Host Species
Human

Derivation
Phage display library

Type
Human antibody

Specificity
Human

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Western Blot

Related Disease
HER2-positive breast cancer

Target

Alternative Names
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;

Gene ID
2064

UniProt ID
P04626

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Breast cancer biomarkers at key points during disease progression

Robust HER2 Detection

The Anti-HER2/neu Recombinant Antibody worked flawlessly in our Western Blot experiments. We were able to detect the overexpression of HER2 in various breast cancer cell lines with high sensitivity. The signal-to-noise ratio was excellent, allowing for clear quantification of protein levels. It has become a standard reagent in our lab for studying ErbB2 signaling pathways.

Great for Research

We utilized this antibody to study the heterodimerization of HER2 with other EGFR family members. It showed high specificity for the target antigen and minimal cross-reactivity. The fact that it is a human recombinant antibody was advantageous for our assay design. We are very satisfied with the quality and reliability of this product for our oncology research.

Q&As

What is the primary application for this HER2 antibody?

A: This Anti-HER2/neu Recombinant Antibody is validated for Western Blot (WB) applications. It is designed to detect Human HER2 (ERBB2) protein in various samples, such as cell lysates from breast cancer lines.
Is this a human or mouse antibody?

A: This is a Recombinant Human antibody. It is derived from a human antibody phage display library and expressed in CHO cells, making it a fully human IgG molecule.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-055CT.pdf

MSDS

MSDS - TAB-055CT.pdf

COA

Certificate of Analysis Lookup

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