This monoclonal antibody can specifically bind to human Prominin-1 (CD133). It also shows a potential effect on tumor growth inhibition including head and neck cancer, glioblastoma and breast cancer.
Figure 1 Specificity of clone 7.
Cells were incubated with either clone 7 or 293C3 (Militenyi) primary antibody, followed by incubation with a labeled secondary antibody, and then analyzed by flow cytometry. The newly developed anti-CD133 clone 7 (open black histogram) stains very specifically the CD133-overexpressing cells, Caco-2 (iii), GBM6 (iv) and U87 cells transfected with CD133 (ii) but not CD133 negative U87 cells (i). Immunostaining of clone 7 is compared to clone 293C3 (open grey histogram). Isotype control is represented by solid grey histogram.
Figure 2 Specificity of clone 7.
Tunicamycin mediated glycosylation inhibition: Caco-2 cells were treated with increasing doses of tunicamycin (2.5, 5, 10 and 20 μg/ml) for 3 days. DMSO treated cells or untreated cells served as controls. Cells were lysed and then analyzed by western blotting using clone 7 or GAPDH antibodies.
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