Anti-Human PROM1 Therapeutic Antibody (Clone 7)

Published Data
Datasheet
MSDS
COA

Figure 1 Specificity of clone 7.

Cells were incubated with either clone 7 or 293C3 (Militenyi) primary antibody, followed by incubation with a labeled secondary antibody, and then analyzed by flow cytometry. The newly developed anti-CD133 clone 7 (open black histogram) stains very specifically the CD133-overexpressing cells, Caco-2 (iii), GBM6 (iv) and U87 cells transfected with CD133 (ii) but not CD133 negative U87 cells (i). Immunostaining of clone 7 is compared to clone 293C3 (open grey histogram). Isotype control is represented by solid grey histogram.

Swaminathan, S. K., Olin, M. R., Forster, C. L., Santa Cruz, K. S., Panyam, J., & Ohlfest, J. R. (2010). Identification of a novel monoclonal antibody recognizing CD133. Journal of immunological methods, 361(1-2), 110-115.

Figure 2 Specificity of clone 7.

Tunicamycin mediated glycosylation inhibition: Caco-2 cells were treated with increasing doses of tunicamycin (2.5, 5, 10 and 20 μg/ml) for 3 days. DMSO treated cells or untreated cells served as controls. Cells were lysed and then analyzed by western blotting using clone 7 or GAPDH antibodies.

IHC

Figure 3 Supernatant of hybridoma clone 7 was used in the immunohistochemical analysis of primary glioblastoma and kidney tissue.

Scale bars for panel C represent 100μm (top) and 10 μm (bottom).

Datasheet

MSDS

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Specifications

Applications

Application Notes
Flow cytometry
Primary human glioblastoma GBM6 cells, U87, or Caco-2 cells were washed three times and suspended in 100 μl of PBS. Cells were stained with either CD133/2 (293C3) or 100 μl of clone 7 hybridoma supernatant, incubated for 30 min at 4 ° C, washed three times, and then suspended in 100 μl of PBS. Cells were further incubated with 10 μl of anti-mouse hylite 647 (1:40 dilution) for 30 min at 4 °C, washed three times, and analyzed by flow cytometry.
5 μm paraffin sections of human kidney or glioblastoma tissue were mounted onto charged slides. The sections were deparaffinized and rehydrated using standard methods, rinsed in running tap-water and placed in a PBS bath for 5 min. The slides were then incubated in a pre-heated steamer bath with staining dish containing EDTA buffer (pH 8.0) for 30 min and then cooled for 20 min. The slides were washed with PBS (pH 7.4) for minimum of 5 min and then subjected to following incubation steps: blocking with serum-free background Sniper (Biocare Medical), primary antibody-1:200 dilution of hybridoma supernatant at 4 °C overnight, Leica Bond post primary, Leica Bond polymer, and DAB chromagen developer. The sections were rinsed well, counterstained with hematoxylin, dehydrated, sealed with a cover slip and imaged.

Target

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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3D IHC3D Immunohistochemistry

ActivActivation

AgonistAgonist

ApopApoptosis

BABioassay

BIBioimaging

BlockBlocking

Cell ScreeningCell Screening

SeparationCell Separation

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CostimCostimulation

CytCytotoxicity

DepletionDepletion

DBDot Blot

EMElectron Microscopy

ELISAEnzyme-linked Immunosorbent Assay

ELISPOTEnzyme-linked Immunosorbent Spot

FCFlow Cytometry

FuncSFunctional Assay

GSGel Super Shift Assay

HAHemagglutination

IAImmunoassay

IBImmunoblotting

ICCImmunocytochemistry

IDImmunodiffusion

IFImmunofluorescence

IHCImmunohistochemistry

IHC-FrImmunohistochemistry-Frozen

IHC-PImmunohistochemistry-Paraffin

REImmunohistology - Resin Sections

IPImmunoprecipitation

IRMAImmunoradiometric Assay

SHIn situ hybridization

InhibInhibition

ICFCIntracellular Staining for Flow Cytometry

KO/KD-WBKnockout/Knockdown target confirmation by Western Blot

Live cell imagingLive cell imaging

CyTOF®Mass Cytometry

MeDIPMethylated DNA Immunoprecipitation

MultiplexMultiplex bead-based assay

NeutNeutralization

PPProtein Purification

PGProteogenomics

RIRadial Immunodiffusion

RIARadioimmunoassay

StimStimulation

SPRSurface Plasmon Resonance

TCTissue Culture

TBTurbidimetry

WBWestern Blot

Anti-Human PROM1 Therapeutic Antibody (Clone 7)