Anti-Human PROM1 Therapeutic Antibody (Clone 7) (CAT#: TAB-1075CL)

This monoclonal antibody can specifically bind to human Prominin-1 (CD133). It also shows a potential effect on tumor growth inhibition including head and neck cancer, glioblastoma and breast cancer.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Published Data
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 Specificity of clone 7.

Cells were incubated with either clone 7 or 293C3 (Militenyi) primary antibody, followed by incubation with a labeled secondary antibody, and then analyzed by flow cytometry. The newly developed anti-CD133 clone 7 (open black histogram) stains very specifically the CD133-overexpressing cells, Caco-2 (iii), GBM6 (iv) and U87 cells transfected with CD133 (ii) but not CD133 negative U87 cells (i). Immunostaining of clone 7 is compared to clone 293C3 (open grey histogram). Isotype control is represented by solid grey histogram.

Swaminathan, S. K., Olin, M. R., Forster, C. L., Santa Cruz, K. S., Panyam, J., & Ohlfest, J. R. (2010). Identification of a novel monoclonal antibody recognizing CD133. Journal of immunological methods, 361(1-2), 110-115.

WB

Figure 2 Specificity of clone 7.

Tunicamycin mediated glycosylation inhibition: Caco-2 cells were treated with increasing doses of tunicamycin (2.5, 5, 10 and 20 μg/ml) for 3 days. DMSO treated cells or untreated cells served as controls. Cells were lysed and then analyzed by western blotting using clone 7 or GAPDH antibodies.

Swaminathan, S. K., Olin, M. R., Forster, C. L., Santa Cruz, K. S., Panyam, J., & Ohlfest, J. R. (2010). Identification of a novel monoclonal antibody recognizing CD133. Journal of immunological methods, 361(1-2), 110-115.

IHC

Figure 3 Supernatant of hybridoma clone 7 was used in the immunohistochemical analysis of primary glioblastoma and kidney tissue.

Scale bars for panel C represent 100μm (top) and 10 μm (bottom).

Swaminathan, S. K., Olin, M. R., Forster, C. L., Santa Cruz, K. S., Panyam, J., & Ohlfest, J. R. (2010). Identification of a novel monoclonal antibody recognizing CD133. Journal of immunological methods, 361(1-2), 110-115.


Specifications

  • Immunogen
  • Recombinant CD133 peptide fragment
  • Host Species
  • Mouse
  • Derivation
  • Phage display library
  • Specificity
  • Human
  • Clone
  • Clone 7
  • Applications
  • WB, IF, FC, IHC
  • Related Disease
  • Head and neck cancer, glioblastoma, breast cancer

Applications

  • Application Notes
  • Flow cytometry
    Primary human glioblastoma GBM6 cells, U87, or Caco-2 cells were washed three times and suspended in 100 μl of PBS. Cells were stained with either CD133/2 (293C3) or 100 μl of clone 7 hybridoma supernatant, incubated for 30 min at 4 ° C, washed three times, and then suspended in 100 μl of PBS. Cells were further incubated with 10 μl of anti-mouse hylite 647 (1:40 dilution) for 30 min at 4 °C, washed three times, and analyzed by flow cytometry.
    5 μm paraffin sections of human kidney or glioblastoma tissue were mounted onto charged slides. The sections were deparaffinized and rehydrated using standard methods, rinsed in running tap-water and placed in a PBS bath for 5 min. The slides were then incubated in a pre-heated steamer bath with staining dish containing EDTA buffer (pH 8.0) for 30 min and then cooled for 20 min. The slides were washed with PBS (pH 7.4) for minimum of 5 min and then subjected to following incubation steps: blocking with serum-free background Sniper (Biocare Medical), primary antibody-1:200 dilution of hybridoma supernatant at 4 °C overnight, Leica Bond post primary, Leica Bond polymer, and DAB chromagen developer. The sections were rinsed well, counterstained with hematoxylin, dehydrated, sealed with a cover slip and imaged.

Target

  • Alternative Names
  • PROM1; prominin 1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "PROM1"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

Send Inquiry

  • Verification code
    Click image to refresh the verification code.
© 2007 - 2020 Creative BioLabs All Rights Reserved
  • 0
  • 0

Cart