This antibody can bind to Prominin-1 which has ADCC activity and CDC activity. It also can potentially work as a pharmaceutical composition fot treatment of tumors.
Figure 1 The MDCK cells stably transfected with human or mouse prominin-1, canine prominin-1-GFP as well as wild type cells (MDCK) were analyzed either by immunocytochemistry using mAbs AC133, AC141, 293C3 and 13A4.
For immunocytochemistry, cells were either cell surface labeled in the cold (A) or permeabilized with saponin after fixation (B). As negative controls, only the secondary antibody was used, and cells were counterstained with DAPI (A, B). Scale bars, 30 μm
Thamm, K., Graupner, S., Werner, C., Huttner, W. B., & Corbeil, D. (2016). Monoclonal antibodies 13A4 and AC133 do not recognize the canine ortholog of mouse and human stem cell antigen prominin-1 (CD133). PloS one, 11(10).
Figure 2 The commercial anti-human and anti-mouse antibodies fail to detect canine prominin-1 by immunoblotting.
(A-C) Detergent lysates prepared from MDCK cells stably transfected with human or mouse prominin-1, canine prominin-1-GFP as well as wild type cells (MDCK) were analyzed by SDS-PAGE under reducing (A, C) and non-reducing (B) conditions and immunoblotting using mAbs AC133, AC141, 293C3, and 13A4 or polyclonal antibody against GFP. β-actin and α-tubulin were used as loading controls. As negative controls, only the secondary antibody (as indicated) was used (C). (D) Lysates from canine prominin-1-GFP transfected cells were incubated with (+) or without (-) PNGase F prior to immunoblotting with anti-GFP antibody or others (as indicated). The arrow and open arrowhead indicate the plasma membrane-associated form and endoplasmic reticulum-associated form of prominin-1, respectively. Asterisk indicates potential disulfide-bridged prominin-1 dimers or multimers, and the black arrowhead shows deglycosylated prominin-1. Molecular mass markers (kDa) are indicated.
Thamm, K., Graupner, S., Werner, C., Huttner, W. B., & Corbeil, D. (2016). Monoclonal antibodies 13A4 and AC133 do not recognize the canine ortholog of mouse and human stem cell antigen prominin-1 (CD133). PloS one, 11(10).
Figure 3 The commercial anti-human and anti-mouse antibodies fail to detect canine prominin-1 by immunoblotting.
Lysates from canine prominin-1-GFP transfected cells were incubated with (+) or without (-) PNGase F prior to immunoblotting with anti-GFP antibody or others. The arrow and open arrowhead indicate the plasma membrane-associated form and endoplasmic reticulum-associated form of prominin-1, respectively. Asterisk indicates potential disulfide-bridged prominin-1 dimers or multimers, and the black arrowhead shows deglycosylated prominin-1. Molecular mass markers (kDa) are indicated.
Thamm, K., Graupner, S., Werner, C., Huttner, W. B., & Corbeil, D. (2016). Monoclonal antibodies 13A4 and AC133 do not recognize the canine ortholog of mouse and human stem cell antigen prominin-1 (CD133). PloS one, 11(10).
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• Confirmed specificity
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B028(A) | Recombinant Anti-human PROM1 Intrabody [(D-Arg)9] | ELISA, Neut, FuncS | scFv-(D-Arg)9 |
IAB-B028(G) | Recombinant Anti-human PROM1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-(+36GFP) |
IAB-B028(T) | Recombinant Anti-human PROM1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G057S | Anti-PROM1 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0375CL-S(P) | Anti-Mouse PROM1 Recombinant Antibody scFv Fragment (293AC1C3H9) | FC | Chimeric (mouse/human) |
TAB-0376CL-S(P) | Anti-Mouse PROM1 Recombinant Antibody scFv Fragment (SDIE 293) | FC | Chimeric (mouse/human) |
TAB-0377CL-S(P) | Anti-Mouse PROM1 Recombinant Antibody scFv Fragment (W6B3H10) | FC | Chimeric (mouse/human) |
TAB-0378CL-S(P) | Anti-Mouse PROM1 Recombinant Antibody scFv Fragment (SDIE W6B3) | FC | Chimeric (mouse/human) |
TAB-0379CL-S(P) | Anti-Mouse PROM1 Recombinant Antibody scFv Fragment | FC | Chimeric (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0183-YC-S(P) | Human Anti-PROM1 Recombinant Antibody (clone h133H/h133La); scFv Fragment | ELISA, FC | Humanized scFv |
HPAB-0088-FY-S(P) | Human Anti-PROM1 Recombinant Antibody; scFv Fragment (HPAB-0088-FY-S(P)) | FC | Human scFv |
HPAB-1396-FY-F(E) | Human Anti-PROM1 Recombinant Antibody (clone H133); scFv Fragment | FC | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0183-YC-F(E) | Human Anti-PROM1 Recombinant Antibody (clone h133H/h133La); Fab Fragment | ELISA, FC | Humanized Fab |
HPAB-0088-FY-F(E) | Human Anti-PROM1 Recombinant Antibody; Fab Fragment (HPAB-0088-FY-F(E)) | FC | Human Fab |
HPAB-1396-FY-S(P) | Human Anti-PROM1 Recombinant Antibody (clone H133); Fab Fragment | FC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-019CN | Mouse Anti-PROM1 Recombinant Antibody (NS-019CN) | ELISA, IF, IHC, FC | Mouse IgG |
NS-019CN-F(E) | Mouse Anti-PROM1 Recombinant Antibody; Fab Fragment (NS-019CN-F(E)) | ELISA, IF, IHC, FC | Mouse Fab |
NS-019CN-S(P) | Mouse Anti-PROM1 Recombinant Antibody; scFv Fragment (NS-019CN-S(P)) | ELISA, IF, IHC, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0088-FY | Human Anti-PROM1 Recombinant Antibody (HPAB-0088-FY) | FC | Human IgG1 |
MRO-0275-CN | Recombinant Mouse Anti-PROM1 Monoclonal Antibody (D2-B1) | IHC, IF | Mouse IgG1 |
VS3-QX919 | Mouse Anti-PROM1 Recombinant Antibody (clone 8F2-H6-D6) | WB | Mouse IgG2b |
VS3-QX920 | Mouse Anti-PROM1 Recombinant Antibody (clone 6H10-F1-C11) | WB | Mouse IgG2a |
VS3-QX921 | Mouse Anti-PROM1 Recombinant Antibody (clone 3F10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-CN1161 | PE-A*02:01/Human PROM1 (YLQWIEFSI) MHC Tetramer | FCM, IHC | |
MHC-CN1162 | APC-A*02:01/Human PROM1 (YLQWIEFSI) MHC Tetramer | FCM, IHC | |
MHC-CN1163 | FITC-A*02:01/Human PROM1 (YLQWIEFSI) MHC Tetramer | FCM, IHC | |
MHC-CN1164 | A*02:01/Human PROM1 (YLQWIEFSI) MHC Tetramer | FCM, IHC | |
MHC-YF689 | A*02:01/Human Prominin1 (YLQWIEFSI) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0060-FY | Rabbit Anti-PROM1 Recombinant Antibody (clone AFY0031) | IF, IHC-P, WB | Rabbit IgG |
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