Recombinant Anti-human SGK1 Intrabody [(D-Arg)9] (CAT#: IAB-B057(A))
Recombinant anti-SGK1 intrabody linked to (D-Arg)9 works within cells to inhibit the activity of human SGK1, expressed in 293T cells. The anti-Sgk intrabodies recognize the enzymatically active form of Sgk and which, upon binding, inhibit Sgk's ability to transphosphorylate.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (HHSteC, RPTEC TERT1, SK-MEL-30, TIME, U-266/84)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
Specifications
- Immunogen
- human serum/glucocorticoid regulated kinase 1
- Host Species
- human
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Products can be used for: Western blot; Neutralization; Functional studies
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SGK1; serum/glucocorticoid regulated kinase 1; SGK; serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1
- Gene ID
- 6446
- UniProt ID
- O00141
Related Resources
mTOR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B057(G) | Recombinant Anti-human SGK1 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
| IAB-B057(T) | Recombinant Anti-human SGK1 Intrabody [Tat] | PCA, Neut, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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