Does this antibody neutralize TNF-alpha?

Yes, the Rat monoclonal antibody (clone MP6-XT22) binds specifically to mouse TNF-alpha and can neutralize its bioactivity (Neut).

Rat Anti-Tnf Recombinant Antibody (clone MP6-XT22)

CAT#: NEUT-2065CQ

This antibody is a rat monoclonal antibody that binds specifically to mouse TNF-α, and it can neutralize the bioactivity of mouse TNF-α.

Gene Expression

Normal Tissue Figure 1 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HDLM-2, NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF

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Specifications

Immunogen
Recombinant mouse TNF-α

Host Species
Rat

Type
Rat IgG1, κ

Specificity
Mouse Tnf

Species Reactivity
Mouse

Clone
MP6-XT22

Applications
ELISA, ICFC, CyTOF, IF, IHC, WB, Neut

Conjugate
Unconjugated

Product Property

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Tnf; tumor necrosis factor; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

Gene ID
21926

UniProt ID
P06804

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Breast cancer biomarkers at key points during disease progression

Neutralizes Mouse TNF-alpha

The clone MP6-XT22 is highly effective at neutralizing the bioactivity of mouse TNF-alpha. We used it in functional assays and saw a dose-dependent inhibition. It is a critical reagent for our inflammation studies involving this cytokine.

Q&As

Does this antibody neutralize TNF-alpha?

A: Yes, the Rat monoclonal antibody (clone MP6-XT22) binds specifically to mouse TNF-alpha and can neutralize its bioactivity (Neut).

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# NEUT-2065CQ, RRID: AB_3714115)

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Related Diseases

Asthma

Inflammatory Bowel Diseases

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NEUT-2065CQ.pdf

MSDS

MSDS - NEUT-2065CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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