Human Anti-Human C5 Rcombinant Antibody (TP-092CL) (CAT#: TP-092CL)
This antiboy is a recombinant humanized monoclonal IgG2/4;κ antibody produced by murine myeloma cell culture and purified by standard bioprocess technology. It contains human constant regions from human IgG2 sequences and human IgG4 sequences and murine complementarity-determining regions grafted onto the human framework light- and heavy-chain variable regions. This antibody is composed of two 448 amino acid heavy chains and two 214 amino acid light chains and has a molecular weight of approximately 148 kDa.
Specific Inquiry
SDS-PAGE
Figure 1 Anti-Human C5 Recombinant Antibody (TP-092CL) in SDS-PAGE
SDS-PAGE analysis of TP-092CL in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 2 Anti-Human C5 Recombinant Antibody (TP-092CL) in WB
Western blot analysis of TP-092CL was performed by loading Human C5 protein.
TP-092CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
ELISA
Figure 3 Anti-Human C5 Recombinant Antibody (TP-092CL) in ELISA
ELISA analysis of TP-092CL was performed by coating with Human C5 protein.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Specifications
- Type
- Biotech
Target
- Function
- Humanized mAb that binds complement protein C5 and inhibits its cleavage to C5a and C5b, preventing the formation of the terminal complement complex C5b–9
Related Resources
Please refer to Eculizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Eculizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
Customer Review
Q&As
Michael Thompson
Excellent for Complement Research
Excellent for Complement Research
The Eculizumab has been a fantastic addition to our complement research. Its specificity and sensitivity are outstanding, providing clear and reliable results. This antibody has become essential for our experiments, improving the accuracy and reliability of our assays.
29-Mar-23
Sarah Harris
Effective and Reliable
Effective and Reliable
We incorporated this antibody into our complement assays, and it has been a great asset. The specificity for complement proteins is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
22-Jun-22
Brian White
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for complement proteins have been particularly beneficial.
30-Aug-21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TP-092CL, RRID: AB_3112052)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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