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  • Mouse Anti-BIRC5 Recombinant Antibody (VS3-CJ139)

Mouse Anti-BIRC5 Recombinant Antibody (VS3-CJ139) (CAT#: VS3-CJ139)

This product is a mouse antibody that recognizes BIRC5.


See more Engineered Antibody products for BIRC5
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4270/if_selected.jpg
Normal Tissue
Figure 2 Testis
Figure 2 Testis
(Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4270/11491_A_6_6.jpg
Figure 3 Bone marrow
Figure 3 Bone marrow
(Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4270/11701_B_4_4.jpg
Figure 4 Lymph node
Figure 4 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4270/11491_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000089685-BIRC5

Specifications

  • Immunogen
  • Recombinant protein corresponding to human Survivin
  • Host Species
  • Mouse
  • Specificity
  • Human, Mouse and Rat BIRC5
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, IHC
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
  • Preservative
  • 0.01% sodium azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:200-1:500).

Target

  • Alternative Names
  • API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin
  • Sequence Similarities
  • Belongs to the IAP family.
  • Cellular Localization
  • Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore
  • Post Translation Modifications
  • Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074).
    Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625).
    Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247).
    Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
    Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
  • Function
  • Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735).
    Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459).
    Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784).
    Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775).
    The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255).
    May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993).
    The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784).
    May play a role in neoplasia (PubMed:10626797).
    Inhibitor of CASP3 and CASP7 (PubMed:21536684).
    Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398).
    Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752).
    Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "BIRC5"

Single-domain Antibody

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NAB-161-VHH Recombinant Anti-human BIRC5 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

Recombinant Antibody

CAT Product Name Application Type
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MOB-0339MZ Mouse Anti-BIRC5 Recombinant Antibody (clone 15D5) IHC Mouse IgG2a
VS3-CJ140 Rabbit Anti-BIRC5 Recombinant Antibody (VS3-CJ140) WB, ICC, IF, IHC, FC Rabbit IgG
VS3-CJ167 Rabbit Anti-BIRC5 Recombinant Antibody (VS3-CJ167) WB, IHC, IP Rabbit IgG
VS4-WK49 Mouse Anti-BIRC5 Recombinant Antibody (VS4-WK49) ELISA, WB, IHC Mouse IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC115 PE-A*24:02/Human Survivin-2B (AYACNTSTL) MHC Tetramer FCM
MHC-LC116 APC-A*24:02/Human Survivin-2B (AYACNTSTL) MHC Tetramer FCM
MHC-LC147 PE-A*02:01/Human Survivin (LMLGEFLKL) MHC Tetramer FCM
MHC-LC148 APC-A*02:01/Human Survivin (LMLGEFLKL) MHC Tetramer FCM
MHC-CN0037 APC-B*35:01/Human Survivin (CPTENEPDY) MHC Tetramer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-045CQ Mouse Anti-BIRC5 Recombinant Antibody (clone 8E2) WB, Neut, ELISA, FC, IF, IHC, IHC-P Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0360 Hi-Affi™ Rabbit Anti-BIRC5 Recombinant Antibody (clone DS360AB) FC, ICC, IHC-P, IP, WB, sELISA Rabbit IgG
MOR-4746 Hi-Affi™ Rabbit Anti-BIRC5 Recombinant Antibody (clone TH260DS) WB, IF, ICC, FC Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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