Mouse Anti-BIRC5 Recombinant Antibody (VS3-CJ139) (CAT#: VS3-CJ139)

• Immunogen
• Recombinant protein corresponding to human Survivin

• Host Species
• Mouse

• Specificity
• Human, Mouse and Rat BIRC5

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:200-1:500).

• Alternative Names
• API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

• Gene ID
• 332

• UniProt ID
• O15392

• Sequence Similarities
• Belongs to the IAP family.

• Cellular Localization
• Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

• Post Translation Modifications
• Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.
  In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074).
  Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625).
  Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247).
  Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.
  Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

• Protein Refseq
• NP_001012270.1; NP_001012271.1; NP_001159.2

• Function
• Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735).
  Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459).
  Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784).
  Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775).
  The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255).
  May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993).
  The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784).
  May play a role in neoplasia (PubMed:10626797).
  Inhibitor of CASP3 and CASP7 (PubMed:21536684).
  Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398).
  Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752).
  Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Select a product category from the dropdown menu below to view related products.