Anti-CD28 Antibody Prodrug, Protease Activated (HuTN228)

Name: Anti-CD28 Antibody Prodrug, Protease Activated (HuTN228)
SKU: VS-1025-YC212
Rating: 5 (1 reviews)

CAT#: VS-1025-YC212

The Anti-CD28 Antibody Prodrug, Protease Activated (HuTN228) is an antibody prodrug comprised of a recombinant human antibody targeting human CD28, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to CD28 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to CD28 effectively. CD28 is the primary co-stimulatory receptor on T-cells, providing the necessary "go" signal for T-cell activation alongside the TCR signal. It is a critical component in CAR T-cell therapy design and the focus of new immunotherapies aimed at boosting T-cell function against tumors.

Gene Expression

Normal Tissue Figure 1 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28

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Specifications

Host Animal
Human

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human CD28

Target
CD28

Species Reactivity
Human

Isotype
IgG

Clone
HuTN228

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein A purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular).

Alternative Names
Tp44; T-cell-specific surface glycoprotein CD28; CD28 molecule

Gene ID
940

UniProt ID
P10747

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-1025-YC212.pdf

MSDS

MSDS - VS-1025-YC212.pdf

COA

Certificate of Analysis Lookup

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CAT
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HPAB-0132CQ-F(E)	Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment	ELISA, IHC, Block	Human Fab
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TAB-066LC	Human Anti-CD28 Recombinant Antibody (TAB-066LC)	FC, IF, ELISA	Human IgG1
TAB-069LC	Anti-Human CD28 Recombinant Antibody (HuTN228)	ELISA, FC	Humanized antibody

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MOB-0424ZL	Mouse Anti-CD28 Recombinant Antibody (clone CBLS-209)	FC, IP, WB, IHC, FuncS	Mouse IgG1
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FAMAB-0013-CN-S(P)	Mouse Anti-CD28 Recombinant Antibody (clone AV7); scFv Fragment	FC	Mouse scFv
HPAB-0132CQ-S(P)	Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment	ELISA, IHC, Block	Human scFv
HPAB-0245-FY-S(P)	Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P))	WB, IP, IF, FC	Mouse scFv
HPAB-0246-FY-S(P)	Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0246-FY-S(P))	FC, FuncS, ICC, IHC, IP, WB	Mouse scFv

CAT	Product Name	Application	Type
TAB-068LC	Anti-Human CD28 Recombinant Antibody (ChTN228)	ELISA, FC	Chimeric antibody (mouse/human)
TAB-068LC-S(P)	Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228)	FC, ELISA	Chimeric antibody (mouse/human)
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PABX-031-S (P)	Recombinant Mouse Anti-CD28 Antibody scFv Fragment (PABX-031-S (P))	WB, ELISA, FuncS	scFv

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FAMAB-0320YC-VH	Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH)	ELISA	Humanized VH
FAMAB-0321YC-VH	Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH)	ELISA	Humanized VH

CAT	Product Name	Application	Type
AFC-TAB-H47	Afuco™ Anti-CD28 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H47)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0724-XY9	Human Anti-CD28 Agonistic Antibody (VS-0724-XY9)	ELISA, Agonistic assays	Human IgG1
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VS-0724-XY11	Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11)	FC, Agonistic assays, CyTOF®	Rat IgG1
VS-0724-XY12	Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12)	FC, IF, IHC, Costim, Agonistic assays	Rabbit IgG, kappa
VS-0724-XY13	Mouse Anti-Cd28 Agonistic Antibody (clone JJ316)	FC, IF, IHC, Costim, Agonistic assays	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS-0424-XY52	AbPlus™ Anti-CD28 Magnetic Beads (VS-0424-XY52)	IP, Protein Purification

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VS-0225-XY33	CytoStream™ Mouse Anti-CD28 Recombinant Antibody (VS-0225-XY33)	FC	Mouse IgG1, kappa

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VS-0325-FY106	Human Anti-CD28 (clone Theralizumab) scFv-Fc Chimera	IA	Human IgG1, scFv-Fc

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