Human Anti-CD28 Recombinant Antibody (clone FR255734)
CAT#: HPAB-0132CQ
This product is a recombinant human antibody that recognizes CD28. FR255734 is a humanized IgG2κ anti-human CD28 antibody that has the complementary determining regions of the mouse anti-human monoclonal antibody TN228 and the Fc domain of human IgG2M3 in which two amino acid mutations (V234A, G237A) have been introduced into the human γ2 chain to eliminate binding of the antibody to FcγR. FR255734 does not cross-react with mouse CD28.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Immunogen
- Human CD28-transfected mouse fibroblast L cells, and fusing immune splenocytes with P3 U1 myeloma cells
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG2M3, κ
- Specificity
- Human CD28
- Species Reactivity
- Human
- Clone
- FR255734
- Applications
- ELISA, IHC, Block, FuncS
- Related Disease
- Psoriasis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition and Functional Assay. Please see the Published Data for further information.
Target
REVIEWS AND Q&AS
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Detailed Signaling Insights
The specificity of FR255734 for CD28 allowed us to gain precise insights into T-cell signaling pathways. Our understanding of T-cell activation has deepened significantly.
Significant Cancer Research Impact
FR255734 has greatly impacted our cancer research by enabling the study of CD28-mediated T-cell responses against tumors, contributing valuable data for immunotherapy development.
Essential for Autoimmunity Research
FR255734 has been essential in our research on autoimmune diseases. It helped us explore CD28 signaling's role in autoimmunity, providing valuable insights into disease mechanisms.
Q&As
-
In order to preserve the action of the FR255734 antibody, what storage conditions are advised?
A: The antibody should be stored at -20°C or lower. Aliquoting upon initial use and avoiding repeated freeze-thaw cycles ensures its stability and functionality over extended periods.
-
Is it possible to use this antibodies on mice to study CD28?
A: No, FR255734 does not cross-react with mouse CD28.
-
Is it possible to utilize the FR255734 antibody in studies on autoimmune diseases?
A: Yes, FR255734 can help explore the role of T-cell co-stimulation in autoimmune diseases, offering insights into how CD28 signaling contributes to the pathology of autoimmune conditions.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone FR255734"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0424ZL | Mouse Anti-CD28 Recombinant Antibody (clone CBLS-209) | FC, IP, WB, IHC, FuncS | Mouse IgG1 |
| HPAB-0078-YJ | Mouse Anti-Cd28 Recombinant Antibody (clone JJ-316) | Costim, Agonist, FC, IF, IHC, FuncS | Mouse IgG1, κ |
| HPAB-0079-YJ | Mouse Anti-Cd28 Recombinant Antibody (clone JJ-319) | IP, FC | Mouse IgG1, κ |
| VS7-HM499 | Mouse Anti-CD28 Recombinant Antibody (clone 3F4A5 ) | ICC, FCM | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-064LC | Anti-Human CD28 Recombinant Antibody (TGN1412) | FC, Stim | Humanized antibody |
| TAB-065LC | Human Anti-CD28 Recombinant Antibody (TAB-065LC) | ELISA | Human IgG, κ |
| TAB-066LC | Human Anti-CD28 Recombinant Antibody (TAB-066LC) | FC, IF, ELISA | Human IgG1 |
| TAB-069LC | Anti-Human CD28 Recombinant Antibody (HuTN228) | ELISA, FC | Humanized antibody |
| TAB-064LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (TGN1412) | FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031 | Recombinant Mouse Anti-CD28 Antibody (PABX-031) | WB, ELISA, FuncS, Activ | IgG |
| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (PABX-031-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (PABX-031-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
| NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
| MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH) | ELISA | Humanized VH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013-CN-S(P) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); scFv Fragment | FC | Mouse scFv |
| HPAB-0132CQ-S(P) | Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment | ELISA, IHC, Block | Human scFv |
| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody Fab Fragment (HPAB-1489-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-1589WJ-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (clone 2E12) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H47) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY9 | Human Anti-CD28 Agonistic Antibody (VS-0724-XY9) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY10 | Mouse Anti-CD28 Agonistic Antibody (VS-0724-XY10) | WB, Agonistic assays | Mouse IgG1 |
| VS-0724-XY11 | Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11) | FC, Agonistic assays, CyTOF® | Rat IgG1 |
| VS-0724-XY12 | Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12) | FC, IF, IHC, Costim, Agonistic assays | Rabbit IgG, kappa |
| VS-0724-XY13 | Mouse Anti-Cd28 Agonistic Antibody (clone JJ316) | FC, IF, IHC, Costim, Agonistic assays | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY52 | AbPlus™ Anti-CD28 Magnetic Beads (VS-0424-XY52) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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