Human Anti-CD28 Recombinant Antibody (clone FR255734) (CAT#: HPAB-0132CQ)
This product is a recombinant human antibody that recognizes CD28. FR255734 is a humanized IgG2κ anti-human CD28 antibody that has the complementary determining regions of the mouse anti-human monoclonal antibody TN228 and the Fc domain of human IgG2M3 in which two amino acid mutations (V234A, G237A) have been introduced into the human γ2 chain to eliminate binding of the antibody to FcγR. FR255734 does not cross-react with mouse CD28.
Specific Inquiry
Inhib
Figure 1 FR255734 inhibits T-cell proliferation and cytokine production.
Humanized, Fc-silent anti-CD28 antibody inhibited T-cell proliferation in a dosedependent manner. T cells were cultured with antihuman CD3 and CD28 antibody and human CD80/P815.
Raychaudhuri, S. P., Kundu-Raychaudhuri, S., Tamura, K., Masunaga, T., Kubo, K., Hanaoka, K.,... & Herzenberg, L. A. (2008). FR255734, a humanized, Fc-Silent, Anti-CD28 antibody, improves psoriasis in the SCID mouse-psoriasis xenograft model. Journal of Investigative Dermatology, 128(8), 1969-1976.
FuncS
Figure 2 ICAM-1 expression in pre- and post-treated biopsies following treatment with FR255734, 10 mg/kg per week for 4 weeks.
Pretreatment (a) and post-treated (b) tissues were stained for ICAM-1 with a rabbit anti-human polyclonal antibody and counterstained with hematoxylin. Post-treated (b) graft demonstrated significant thinning of the epidermis and marked reduction of ICAM-1 in the blood vessels. Brown positive cells marked by black arrows indicate expression of ICAM-1 on the luminal endothelial cells on both sides of a blood vessel extending from deep dermis to the papillary dermis.
Raychaudhuri, S. P., Kundu-Raychaudhuri, S., Tamura, K., Masunaga, T., Kubo, K., Hanaoka, K.,... & Herzenberg, L. A. (2008). FR255734, a humanized, Fc-Silent, Anti-CD28 antibody, improves psoriasis in the SCID mouse-psoriasis xenograft model. Journal of Investigative Dermatology, 128(8), 1969-1976.
Specifications
- Immunogen
- Human CD28-transfected mouse fibroblast L cells, and fusing immune splenocytes with P3 U1 myeloma cells
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG2M3, κ
- Specificity
- Human CD28
- Species Reactivity
- Human
- Clone
- FR255734
- Applications
- ELISA, IHC, Block, FuncS
- Related Disease
- Psoriasis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition and Functional Assay. Please see the Published Data for further information.
Target
- Alternative Names
- CD28 Molecule; CD28 Antigen; Tp44; T-Cell-Specific Surface Glycoprotein CD28; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44);
- Gene ID
- 940
- UniProt ID
- P10747
Related Resources
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Single-domain Antibody
Recombinant Antibody
ADCC Enhanced Antibody
Agonistic Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-981 | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment | Agonist, Stim, Neu, FC | Mouse IgG1 F(ab')2, κ |
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
| HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
-
Detailed Signaling Insights
The specificity of FR255734 for CD28 allowed us to gain precise insights into T-cell signaling pathways. Our understanding of T-cell activation has deepened significantly. -
Significant Cancer Research Impact
FR255734 has greatly impacted our cancer research by enabling the study of CD28-mediated T-cell responses against tumors, contributing valuable data for immunotherapy development. -
Essential for Autoimmunity Research
FR255734 has been essential in our research on autoimmune diseases. It helped us explore CD28 signaling's role in autoimmunity, providing valuable insights into disease mechanisms.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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