SmartAb™ Recombinant Anti-CD28 pH-dependent Antibody (Clone Lulizumab)
CAT#: VS-0825-YC49
The anti-CD28 pH-dependent antibody (clone Lulizumab) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human CD28. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for CD28 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from CD28 at pH 6.0. This pH-sensitive antibody retains its binding affinity for CD28 on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human CD28 is a critical co-stimulatory receptor expressed on T-cells that provides the second signal required for their full activation. It binds to B7-1 (CD80) and B7-2 (CD86) on antigen-presenting cells, which leads to T-cell proliferation and cytokine production.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human V-kappa
- Specificity
- Human CD28
- Species Reactivity
- Human
- Clone
- Lulizumab
- Applications
- ELISA, IF, IHC, IP, FC, Neut
Product Property
- Clonality
- Monoclonal
- Purification
- Protein L purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6) | WB, ELISA, FuncS | Mouse IgG1 |
| HPAB-0245-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0245-FY) | WB, IP, IF, FC | Mouse IgG1 |
| HPAB-0246-FY | Mouse Anti-CD28 Recombinant Antibody (HPAB-0246-FY) | FC, FuncS, ICC, IHC, IP, WB | Mouse IgG1 |
| HPAB-1589WJ | Mouse Anti-CD28 Recombinant Antibody (clone 2E12) | ELISA, WB | Mouse IgG |
| VS-0725-YC261 | Mouse Anti-CD28 Recombinant Antibody | FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-0246-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0246-FY-F(E)) | FC, FuncS, ICC, IHC, IP, WB | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody scFv Fragment (HPAB-1489-FY-S(P)) | WB, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-064LC | Anti-Human CD28 Recombinant Antibody (TGN1412) | FC, Stim | Humanized antibody |
| TAB-065LC | Human Anti-CD28 Recombinant Antibody (TAB-065LC) | ELISA | Human IgG, κ |
| TAB-066LC | Human Anti-CD28 Recombinant Antibody (TAB-066LC) | FC, IF, ELISA | Human IgG1 |
| TAB-069LC | Anti-Human CD28 Recombinant Antibody (HuTN228) | ELISA, FC | Humanized antibody |
| TAB-064LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (TGN1412) | FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031 | Recombinant Mouse Anti-CD28 Antibody (PABX-031) | WB, ELISA, FuncS, Activ | IgG |
| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (PABX-031-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (PABX-031-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
| NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
| NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
| NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
| MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH) | ELISA | Humanized VH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013-CN-S(P) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); scFv Fragment | FC | Mouse scFv |
| HPAB-0132CQ-S(P) | Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment | ELISA, IHC, Block | Human scFv |
| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody Fab Fragment (HPAB-1489-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1144WJ-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-1144WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-1589WJ-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (clone 2E12) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H47) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY9 | Human Anti-CD28 Agonistic Antibody (VS-0724-XY9) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY10 | Mouse Anti-CD28 Agonistic Antibody (VS-0724-XY10) | WB, Agonistic assays | Mouse IgG1 |
| VS-0724-XY11 | Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11) | FC, Agonistic assays, CyTOF® | Rat IgG1 |
| VS-0724-XY12 | Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12) | FC, IF, IHC, Costim, Agonistic assays | Rabbit IgG, kappa |
| VS-0724-XY13 | Mouse Anti-Cd28 Agonistic Antibody (clone JJ316) | FC, IF, IHC, Costim, Agonistic assays | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY52 | AbPlus™ Anti-CD28 Magnetic Beads (VS-0424-XY52) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY88 | Mouse Anti-NHP CD28 Recombinant Antibody (VS-1024-XY88) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY33 | CytoStream™ Mouse Anti-CD28 Recombinant Antibody (VS-0225-XY33) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY106 | Human Anti-CD28 (clone Theralizumab) scFv-Fc Chimera | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC69 | Recombinant Anti-CD28 Vesicular Antibody, EV Displayed (VS-0425-YC69) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1157 | Anti-CD28 Immunohistochemistry Kit | IHC |
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