Mouse Anti-Cd28 Agonistic Antibody (clone JJ316)

Name: Mouse Anti-Cd28 Agonistic Antibody (clone JJ316)
SKU: VS-0724-XY13
Rating: 5 (1 reviews)

CAT#: VS-0724-XY13

This product is an agonistic mouse antibody that recognizes Cd28 (Cd28 molecule), which involves in T cell activation and T cell costimulation. It was made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells. This antibody specifically recognizes rat Cd28. It has been used in a variety of applications, such as Flow cytometry, Immunofluorescence, Immunohistochemistry, Costimulation or Agonistic assays.

Gene Expression

Normal Tissue Figure 1 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28

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Specifications

Immunogen
Rat CD28 cDNA-transfected A20/J cells

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
Cd28

Species Reactivity
Rat

Clone
JJ316

Applications
FC, IF, IHC, Costim, Agonistic assays

Product Property

Purification
Protein G purified

Purity
>95%, determined by SDS-PAGE

Buffer
PBS

Preservative
0.02 % Proclin 300

Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target

Alternative Names
CD28RNA

Gene ID
25660

UniProt ID
P31042

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Related Diseases

Rheumatoid Arthritis

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Maturity Onset Diabetes of the Young

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0724-XY13.pdf

MSDS

MSDS - VS-0724-XY13.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Recommended Dilution Buffer

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Secondary Antibody

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