Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11)

Name: Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11)
SKU: VS-0724-XY11
Rating: 5 (1 reviews)

CAT#: VS-0724-XY11

It is an agonistic rat antibody specific for Cd28, which enables protein kinase binding activity. The antibody specifically targets mouse Cd28. This product has been tested using Flow cytometry, Agonistic assays and Mass Cytometry. The recommended dilutions of Flow cytometry and Agonistic assays is 0.25 µg/10⁶ cells and 0.07-0.42 µg/mL.

Gene Expression

Normal Tissue Figure 1 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28

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Specifications

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD28

Host Species
Rat

Type
Rat IgG1

Specificity
Cd28

Species Reactivity
Mouse

Applications
FC, Agonistic assays, CyTOF®

Product Property

Purification
Protein A or G purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS

Preservative
No preservatives

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
FC: 0.25 µg/10⁶ cells
Agonist: 0.07-0.42 µg/mL

Target

Alternative Names
Cd28 antigen

Gene ID
12487

UniProt ID
P31041

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0724-XY11.pdf

MSDS

MSDS - VS-0724-XY11.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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