Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment (CAT#: HPAB-0132CQ-F(E))
This product is a recombinant human antibody Fab fragment that recognizes CD28. FR255734 is a humanized IgG2κ anti-human CD28 antibody that has the complementary determining regions of the mouse anti-human monoclonal antibody TN228 and the Fc domain of human IgG2M3 in which two amino acid mutations (V234A, G237A) have been introduced into the human γ2 chain to eliminate binding of the antibody to FcγR. FR255734 does not cross-react with mouse CD28.
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Normal Tissue
RNA Expression
Figure 1 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Figure 2 Tonsil
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
Specifications
- Immunogen
- Human CD28-transfected mouse fibroblast L cells, and fusing immune splenocytes with P3 U1 myeloma cells
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human Fab
- Specificity
- Human CD28
- Species Reactivity
- Human
- Clone
- FR255734
- Applications
- ELISA, IHC, Block
- Related Disease
- Psoriasis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD28 Molecule; CD28 Antigen; Tp44; T-Cell-Specific Surface Glycoprotein CD28; T-Cell-Specific Surface Glycoprotein; CD28 Antigen (Tp44);
- Gene ID
- 940
- UniProt ID
- P10747
Related Resources
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-981 | Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment | Agonist, Stim, Neu, FC | Mouse IgG1 F(ab')2, κ |
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody scFv Fragment (HPAB-1489-FY-S(P)) | WB, ELISA | Human scFv |
| HPAB-1589WJ-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (clone 2E12) | ELISA, WB | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Precise Signaling Insights
Using the FR255734 Fab fragment allowed us to precisely study CD28-mediated signaling without Fc receptor interactions. This specificity enhanced the accuracy of our findings on T-cell activation.
Effective Controls
Implementing appropriate controls with the FR255734 antibody ensured accurate detection of T-cell activation. The controls emphasized the antibody's specific effects and improved our data quality.
Flexible Titration
Titrating the FR255734 antibody over a range of concentrations allowed us to determine the optimal dose for our in vitro studies. This flexibility facilitated precise tuning of T-cell activation.
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For Research Use Only. Not For Clinical Use.
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