Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment (CAT#: HPAB-0132CQ-F(E))

• Precise Signaling Insights
  Using the FR255734 Fab fragment allowed us to precisely study CD28-mediated signaling without Fc receptor interactions. This specificity enhanced the accuracy of our findings on T-cell activation.
• Effective Controls
  Implementing appropriate controls with the FR255734 antibody ensured accurate detection of T-cell activation. The controls emphasized the antibody's specific effects and improved our data quality.
• Flexible Titration
  Titrating the FR255734 antibody over a range of concentrations allowed us to determine the optimal dose for our in vitro studies. This flexibility facilitated precise tuning of T-cell activation.

1. Q 1: Could one use the Human Anti-CD28 Fab (FR255734) to look at mechanisms leading to T-cell co-stimulation?

   A: Yes, the FR255734 clone targets CD28, a critical co-stimulatory molecule on T-cells, enabling in-depth studies on T-cell activation and proliferation in co-stimulation pathways.

2. Q 2: Is it possible to investigate cytokine production after T-cell activation using the FR255734 clone?

   A: Yes, the FR255734 Fab fragment can be used to stimulate T-cells via CD28, allowing researchers to measure subsequent cytokine production using assays like ELISA or multiplex cytokine profiling.

3. Q 3: Is it appropriate to utilize the FR255734 antibody in humanized mouse models for preclinical research?

   A: While the FR255734 antibody is human-specific, it can be employed in humanized mouse models to study human T-cell responses, providing insights into CD28-mediated T-cell co-stimulation in a preclinical setting.

View the frequently asked questions answered by Creative Biolabs Support.