Recombinant Mouse Antibody binds selectively to Mouse CD28, expressed in HPB-M.
Figure 1 The CD28 superagonist ANC28.1 induces polyclonal T cell activation in vitro and leads to ZAP70 independent phosphorylation of LAT and PLCc1.
5x10⁴ freshly prepared human T cells per well were seeded in 96-well plates and treated with the indicated combinations of antibodies or were incubated in medium as a control.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Figure 2 Induction of sustained calcium flux by human CD28 superagonistic antibody ANC28.1.
1x10⁶ freshly prepared Indo-1 loaded human T cells were stimulated with either a 1:50 v/v dilution of ascites fluid of CD3 mAb MEM92 (orange triangle), 10 mg/ml conventional CD28 mAb (CD28.2; blue triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle) or 10 mg/ml ANC28.1 (red triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle).
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Figure 3 Sustained calcium flux, T cell proliferation, and T cell signaling upon TGN1412 treatment.
5x10⁴ freshly prepared human T-cells/96-well were stimulated with the indicated concentrations of ANC28.1 or TGN1412 or were left untreated as a control. For crosslinking of ANC28.1, a polyclonal goat anti-mouse antiserum was used and crosslinking of TGN1412 was performed.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Figure 4 Only engagement of CD81 by HCV-E2 or neutralizing Abs synergizes with ligation of CD28.
Human naive T cells were cultured with recombinant HCV envelope proteins immobilized on plate-bound anti-E2 plus soluble anti-CD28 (clone ANC28.1/5D10).
Serra, A., Nuti, S., Tavarini, S., Sammicheli, C., Rosa, D., Saletti, G., ... & Wack, A. (2008). Coligation of the hepatitis C virus receptor CD81 with CD28 primes naive T lymphocytes to acquire type 2 effector function. The Journal of Immunology, 181(1), 174-185.
Figure 5 ANC28 has properties of 'mitogenic' CD28 antibody.
CFSE-labeled PBMC stimulated with CD3, PHA, ANC28 or 28.2 alone, or CD28 mAb in combination with CD3 were analyzed for activation (CD25 up-regulation) and expansion (CFSE dye dilution) by flow cytometry on day 6.
Singh, M., Basu, S., Camell, C., Couturier, J., Nudelman, R. J., Medina, M. A., ... & Lewis, D. E. (2008). Selective expansion of memory CD4+ T cells by mitogenic human CD28 generates inflammatory cytokines and regulatory T cells. European journal of immunology, 38(6), 1522-1532.
Figure 6 Selective expansion of CD4+CD45RO+ cells by ANC28.
CFSE-labeled purified CD4⁺, CD8⁺ cells stimulated with 28.2 or ANC28.
Singh, M., Basu, S., Camell, C., Couturier, J., Nudelman, R. J., Medina, M. A., ... & Lewis, D. E. (2008). Selective expansion of memory CD4+ T cells by mitogenic human CD28 generates inflammatory cytokines and regulatory T cells. European journal of immunology, 38(6), 1522-1532.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H46 | Humanized Anti-CD28 Recombinant Antibody (clone Lulizumab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | Humanized V-kappa |
TAB-064LC | Anti-Human CD28 Recombinant Antibody (TGN1412) | FC, Stim | Humanized antibody |
TAB-065LC | Human Anti-CD28 Recombinant Antibody (TAB-065LC) | ELISA | Human IgG, κ |
TAB-066LC | Human Anti-CD28 Recombinant Antibody (TAB-066LC) | FC, IF, ELISA | Human IgG1 |
TAB-069LC | Anti-Human CD28 Recombinant Antibody (HuTN228) | ELISA, FC | Humanized antibody |
There are currently no Customer reviews or questions for DrMAB-981. Click the button above to contact us or submit your feedback about this product.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.