Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment (CAT#: DrMAB-981)
Recombinant Mouse Antibody binds selectively to Mouse CD28, expressed in HPB-M.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 The CD28 superagonist ANC28.1 induces polyclonal T cell activation in vitro and leads to ZAP70 independent phosphorylation of LAT and PLCc1.
5x10⁴ freshly prepared human T cells per well were seeded in 96-well plates and treated with the indicated combinations of antibodies or were incubated in medium as a control.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Figure 2 Induction of sustained calcium flux by human CD28 superagonistic antibody ANC28.1.
1x10⁶ freshly prepared Indo-1 loaded human T cells were stimulated with either a 1:50 v/v dilution of ascites fluid of CD3 mAb MEM92 (orange triangle), 10 mg/ml conventional CD28 mAb (CD28.2; blue triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle) or 10 mg/ml ANC28.1 (red triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle).
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Figure 3 Sustained calcium flux, T cell proliferation, and T cell signaling upon TGN1412 treatment.
5x10⁴ freshly prepared human T-cells/96-well were stimulated with the indicated concentrations of ANC28.1 or TGN1412 or were left untreated as a control. For crosslinking of ANC28.1, a polyclonal goat anti-mouse antiserum was used and crosslinking of TGN1412 was performed.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Specifications
- Immunogen
- Recombinant Human CD28 and HPB-MLT Human tumor cells
- Host Species
- Mouse
- Type
- Mouse IgG1 F(ab')2, κ
- Specificity
- Antibody recognizes CD28 expressed on T cells and also on BHK cells transfected with Human CD28. This antibody has been characterized as a superagonist.
- Species Reactivity
- Human
- Clone
- ANC28.1/5D10
- Applications
- Agonist, Stim, Neu, FC
Product Property
- Purification
- Protein A purification
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- 1 mg/ml
- Buffer
- 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CD28 antibody has been reported in applications of Agonist, Stimulation, Neutralization, Flow Cytometry.
Agonist: 10 μg/ml.
Stim: 10 mg/ml.
Target
- Alternative Names
- CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
- Gene ID
- 940
- UniProt ID
- P31041
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody scFv Fragment (HPAB-1489-FY-S(P)) | WB, ELISA | Human scFv |
HPAB-1144WJ-F(E) | Human Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-1144WJ-F(E)) | ELISA | Humanized Fab |
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