Mouse Anti-CD28 Recombinant Antibody (clone ANC28.1/5D10); F(ab')2 Fragment
CAT#: DrMAB-981
Recombinant Mouse Antibody binds selectively to Mouse CD28, expressed in HPB-M.
Published Data
Gene Expression
Agonist
Figure 1 The CD28 superagonist ANC28.1 induces polyclonal T cell activation in vitro and leads to ZAP70 independent phosphorylation of LAT and PLCc1.
5x10⁴ freshly prepared human T cells per well were seeded in 96-well plates and treated with the indicated combinations of antibodies or were incubated in medium as a control.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Stim
Figure 2 Induction of sustained calcium flux by human CD28 superagonistic antibody ANC28.1.
1x10⁶ freshly prepared Indo-1 loaded human T cells were stimulated with either a 1:50 v/v dilution of ascites fluid of CD3 mAb MEM92 (orange triangle), 10 mg/ml conventional CD28 mAb (CD28.2; blue triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle) or 10 mg/ml ANC28.1 (red triangle) followed by crosslinking with 20 mg/ml polyclonal goat anti-mouse antiserum (black triangle).
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
Stim
Figure 3 Sustained calcium flux, T cell proliferation, and T cell signaling upon TGN1412 treatment.
5x10⁴ freshly prepared human T-cells/96-well were stimulated with the indicated concentrations of ANC28.1 or TGN1412 or were left untreated as a control. For crosslinking of ANC28.1, a polyclonal goat anti-mouse antiserum was used and crosslinking of TGN1412 was performed.
Waibler, Z., Sender, L. Y., Merten, C., Hartig, R., Kliche, S., Gunzer, M., ... & Schraven, B. (2008). Signaling signatures and functional properties of anti-human CD28 superagonistic antibodies. PloS one, 3(3).
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Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Immunogen
- Recombinant Human CD28 and HPB-MLT Human tumor cells
- Host Species
- Mouse
- Type
- Mouse IgG1 F(ab')2, κ
- Specificity
- Antibody recognizes CD28 expressed on T cells and also on BHK cells transfected with Human CD28. This antibody has been characterized as a superagonist.
- Species Reactivity
- Human
- Clone
- ANC28.1/5D10
- Applications
- Agonist, Stim, Neu, FC
Product Property
- Purification
- Protein A purification
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- 1 mg/ml
- Buffer
- 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CD28 antibody has been reported in applications of Agonist, Stimulation, Neutralization, Flow Cytometry.
Agonist: 10 μg/ml.
Stim: 10 mg/ml.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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See other products for "Clone ANC28.1/5D10"
- CAT
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| HPAB-0245-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P)) | WB, IP, IF, FC | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
| HPAB-0246-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0246-FY-F(E)) | FC, FuncS, ICC, IHC, IP, WB | Mouse Fab |
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|---|---|---|---|
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| TAB-065LC | Human Anti-CD28 Recombinant Antibody (TAB-065LC) | ELISA | Human IgG, κ |
| TAB-066LC | Human Anti-CD28 Recombinant Antibody (TAB-066LC) | FC, IF, ELISA | Human IgG1 |
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| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH) | ELISA | Humanized VH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY9 | Human Anti-CD28 Agonistic Antibody (VS-0724-XY9) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY10 | Mouse Anti-CD28 Agonistic Antibody (VS-0724-XY10) | WB, Agonistic assays | Mouse IgG1 |
| VS-0724-XY11 | Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11) | FC, Agonistic assays, CyTOF® | Rat IgG1 |
| VS-0724-XY12 | Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12) | FC, IF, IHC, Costim, Agonistic assays | Rabbit IgG, kappa |
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