Human Anti-CD28 Recombinant Antibody (clone FR255734); scFv Fragment
CAT#: HPAB-0132CQ-S(P)
This product is a recombinant human antibody scFv fragment that recognizes CD28. FR255734 is a humanized IgG2κ anti-human CD28 antibody that has the complementary determining regions of the mouse anti-human monoclonal antibody TN228 and the Fc domain of human IgG2M3 in which two amino acid mutations (V234A, G237A) have been introduced into the human γ2 chain to eliminate binding of the antibody to FcγR. FR255734 does not cross-react with mouse CD28.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_9_8.jpg
Normal Tissue
Figure 2 Tonsil
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70003/161787_A_6_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178562-CD28
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Specifications
- Immunogen
- Human CD28-transfected mouse fibroblast L cells, and fusing immune splenocytes with P3 U1 myeloma cells
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human scFv
- Specificity
- Human CD28
- Species Reactivity
- Human
- Clone
- FR255734
- Applications
- ELISA, IHC, Block
- Related Disease
- Psoriasis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Related Diseases
Rheumatoid Arthritis
Systemic Lupus Erythematosus
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone FR255734"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0424ZL | Mouse Anti-CD28 Recombinant Antibody (clone CBLS-209) | FC, IP, WB, IHC, FuncS | Mouse IgG1 |
| PABL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6) | WB, ELISA, FuncS | Mouse IgG1 |
| MOB-0354MZ | Mouse Anti-CD28 Recombinant Antibody (clone DD29.1) | ELISA | Mouse IgG1 |
| HPAB-1144WJ | Human Anti-CD28 Recombinant Antibody (HPAB-1144WJ) | ELISA | Humanized IgG |
| VS13-YC159 | Rabbit Anti-CD28 Recombinant Antibody (VS13-YC159) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| HPAB-0245-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0245-FY-S(P)) | WB, IP, IF, FC | Mouse scFv |
| HPAB-0246-FY-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (HPAB-0246-FY-S(P)) | FC, FuncS, ICC, IHC, IP, WB | Mouse scFv |
| HPAB-1489-FY-F(E) | Human Anti-CD28 Recombinant Antibody Fab Fragment (HPAB-1489-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1589WJ-S(P) | Mouse Anti-CD28 Recombinant Antibody; scFv Fragment (clone 2E12) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-365 | Mouse Anti-CD28 Recombinant Antibody (clone 1C6); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0013-CN-F(E) | Mouse Anti-CD28 Recombinant Antibody (clone AV7); Fab Fragment | FC | Mouse Fab |
| HPAB-0132CQ-F(E) | Human Anti-CD28 Recombinant Antibody (clone FR255734); Fab Fragment | ELISA, IHC, Block | Human Fab |
| HPAB-0245-FY-F(E) | Mouse Anti-CD28 Recombinant Antibody; Fab Fragment (HPAB-0245-FY-F(E)) | WB, IP, IF, FC | Mouse Fab |
| HPAB-1489-FY-S(P) | Human Anti-CD28 Recombinant Antibody scFv Fragment (HPAB-1489-FY-S(P)) | WB, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-068LC | Anti-Human CD28 Recombinant Antibody (ChTN228) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-068LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| TAB-068LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (ChTN228) | FC, ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-064LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (TGN1412) | FC | Humanized antibody |
| TAB-065LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-065LC-S(P)) | ELISA | Human scFv |
| TAB-066LC-S(P) | Human Anti-CD28 Recombinant Antibody; scFv Fragment (TAB-066LC-S(P)) | ELISA | Human scFv |
| TAB-069LC-S(P) | Anti-Human CD28 Recombinant Antibody scFv Fragment (HuTN228) | FC, ELISA | Humanized antibody |
| TAB-064LC-F(E) | Anti-Human CD28 Recombinant Antibody Fab Fragment (TGN1412) | FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031 | Recombinant Mouse Anti-CD28 Antibody (PABX-031) | WB, ELISA, FuncS, Activ | IgG |
| PABX-031-F (E) | Recombinant Mouse Anti-CD28 Antibody Fab Fragment (PABX-031-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-031-S (P) | Recombinant Mouse Anti-CD28 Antibody scFv Fragment (PABX-031-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-334CQ | Mouse Anti-CD28 Recombinant Antibody (clone CD28.6) | FC, FuncS, Neut, WB | Mouse IgG2a, κ |
| NEUT-337CQ | Hamster Anti-CD28 Recombinant Antibody (clone MAB0818) | Neut | Hamster IgG1 |
| NEUT-338CQ | Hamster Anti-CD28 Recombinant Antibody (clone PV-1) | ELISA, FC, Neut | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-335CQ | Hamster Anti-CD28 Recombinant Antibody (clone 37.51) | IHC-Fr, FC, IP, Block, FuncS | Hamster IgG |
| NEUT-336CQ | Mouse Anti-CD28 Recombinant Antibody (clone E18) | FC, Costim, Block | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4053 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI120DS) | FC | Rabbit IgG |
| MOR-4054 | Hi-Affi™ Rabbit Anti-CD28 Recombinant Antibody (clone SI121DS) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0320YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0320YC-VH) | ELISA | Humanized VH |
| FAMAB-0321YC-VH | Recombinant Humanized Anti-CD28 Single Domain Antibody (FAMAB-0321YC-VH) | ELISA | Humanized VH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H47 | Afuco™ Anti-CD28 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H47) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY9 | Human Anti-CD28 Agonistic Antibody (VS-0724-XY9) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY10 | Mouse Anti-CD28 Agonistic Antibody (VS-0724-XY10) | WB, Agonistic assays | Mouse IgG1 |
| VS-0724-XY11 | Rat Anti-Cd28 Agonistic Antibody (VS-0724-XY11) | FC, Agonistic assays, CyTOF® | Rat IgG1 |
| VS-0724-XY12 | Rabbit Anti-Cd28 Agonistic Antibody (VS-0724-XY12) | FC, IF, IHC, Costim, Agonistic assays | Rabbit IgG, kappa |
| VS-0724-XY13 | Mouse Anti-Cd28 Agonistic Antibody (clone JJ316) | FC, IF, IHC, Costim, Agonistic assays | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY52 | AbPlus™ Anti-CD28 Magnetic Beads (VS-0424-XY52) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY88 | Mouse Anti-NHP CD28 Recombinant Antibody (VS-1024-XY88) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY33 | CytoStream™ Mouse Anti-CD28 Recombinant Antibody (VS-0225-XY33) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY106 | Human Anti-CD28 (clone Theralizumab) scFv-Fc Chimera | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC69 | Recombinant Anti-CD28 Vesicular Antibody, EV Displayed (VS-0425-YC69) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1157 | Anti-CD28 Immunohistochemistry Kit | IHC |
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