Mouse Anti-CD3E Recombinant Antibody (clone 33H1)
CAT#: VS11-QX33
This product is a recombinant mouse antibody that recognizes CD3E. This antibody can be used for the following applications: ELISA, WB, IHC. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line JURKAT
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
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Specifications
- Immunogen
- Recombinant human CD3 protein (amino acids 23-126)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human CD3E
- Species Reactivity
- Human
- Clone
- 33H1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
- Gene ID
- 916
- UniProt ID
- P07766
- Long Name
- T-cell surface glycoprotein CD3 epsilon chain
- Cellular Localization
- Endoplasmic reticulum, Golgi apparatus, Plasma membrane
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G010E | Anti-CD3E immunotoxin UCHT1 (scFv)-PE | Cytotoxicity assay, Function study | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-032 | Recombinant Grey hamster Anti-CD3E Antibody (2C11) | WB, ELISA, FuncS, IP | IgG |
| PABX-033 | Recombinant Grey hamster Anti-CD3E Antibody (17A2) | WB, ELISA, FuncS | IgG |
| PABX-032-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (2C11) | WB, ELISA, FuncS | Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| MOR-0058-FY | Rabbit Anti-CD3E Recombinant Antibody (clone AFY0029) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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