Mouse Anti-CD3E Recombinant Antibody (clone 33H1)

Name: Mouse Anti-CD3E Recombinant Antibody (clone 33H1)
SKU: VS11-QX33
Rating: 5 (1 reviews)

CAT#: VS11-QX33

This product is a recombinant mouse antibody that recognizes CD3E. This antibody can be used for the following applications: ELISA, WB, IHC. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line JURKAT

Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg

Normal Tissue Figure 2 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E

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Specifications

Immunogen
Recombinant human CD3 protein (amino acids 23-126)

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2a, κ

Antibody Isotype
IgG2a, κ

Specificity
Human CD3E

Species Reactivity
Human

Clone
33H1

Applications
ELISA, WB, IHC

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Purity
>95% as determined by SDS-PAGE

Concentration
1 mg/mL (Please refer to the vial label for the specific concentration.)

Buffer
0.2 μm filtered solution in PBS

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipped at 4°C.

Target

Alternative Names
T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain

Gene ID
916

UniProt ID
P07766

Long Name
T-cell surface glycoprotein CD3 epsilon chain

Cellular Localization
Endoplasmic reticulum, Golgi apparatus, Plasma membrane

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS11-QX33.pdf

MSDS

MSDS - VS11-QX33.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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