Rabbit Anti-CDKN2A (p16) Recombinant Antibody (clone 0725W69)
CAT#: VS-0725-FY236
This recombinant antibody, clone 0725W69, targets a peptide from human CDKN2A. It is validated for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). CDKN2A (Cyclin-dependent kinase inhibitor 2A), also known as p16, is a critical negative regulator of normal cell proliferation. This antibody is a valuable tool for studying cell cycle control and its implications in cancer, where it acts as a tumor suppressor.
Specifications
- Immunogen
- A peptide from human CDKN2A.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDKN2A (p16)
- Species Reactivity
- Human
- Clone
- 0725W69
- Applications
- Chromatin Immunoprecipitation-Sequencing, Immunofluorescence, Immunoprecipitation, Western Blot
- Related Disease
- Melanoma
Product Property
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 0.5-2 µg/mL
Immunoprecipitation: 2 µg/mL per Immunoprecipitation
Immunofluorescence: 2 µg per ml
Chromatin Immunoprecipitation-Sequencing: 2 µg per Chromatin Immunoprecipitation-Sequencing
Target
- Alternative Names
- Cyclin Dependent Kinase Inhibitor 2A; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase 4 Inhibitor A; Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumor Suppressor 1; Alternative Reading Frame; P16-INK4A; P16INK4A; P14ARF; CDKN2; CDK4I; MTS-1; MTS1; MLM
- Gene ID
- 1029
- UniProt ID
- P42771
- Long Name
- Cyclin Dependent Kinase Inhibitor 2A
- Sequence Similarities
- Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation seems to increase interaction with CDK4.
- Protein Refseq
- NP_000068.1; NP_001182061.1; NP_478104.2
- Function
- Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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