Rabbit Anti-CEP55 Recombinant Antibody (clone R05-6A6)
CAT#: VS3-FY1986
This is a rabbit recombinant monoclonal antibody that detects human CEP55. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, ICC/IF. The R05-6A6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200).
Tested Data
Gene Expression
WB
Figure 1 Anti-CEP55 Antibody (VS3-FY1986) in WB.
The CEP55 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CEP55 by western blot assay.
ICC
Figure 2 Anti-CEP55 Antibody (VS3-FY1986) in ICC.
Immunocytochemistry staining of CEP55 (green) in Hela using the anti-CEP55 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/1252_H8_3_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/107403_A_8_3.jpg
Normal Tissue
Figure 3 Rectum
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/107403_A_5_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/107403_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/107403_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23430/107403_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138180-CEP55
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Specifications
- Immunogen
- Synthetic peptide of human CEP55
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CEP55
- Species Reactivity
- Human, Rat
- Clone
- R05-6A6
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.
- Related Disease
- Multinucleated Neurons, Anhydramnios, Renal Dysplasia, Cerebellar Hypoplasia, And Hydranencephaly and Meckel Syndrome, Type 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Centrosomal Protein 55; Up-Regulated In Colon Cancer 6; Cancer/Testis Antigen 111; Centrosomal Protein 55kDa; C10orf3; URCC6
- Gene ID
- 55165
- UniProt ID
- Q53EZ4
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436.
Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary.
Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
- Protein Refseq
- XP_011538220.1; XP_011538221.1; XP_011538222.1; XP_016871861.1; XP_016871862.1
- Function
- Plays a role in mitotic exit and cytokinesis.
Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CEP55"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0664 | Hi-Affi™ Rabbit Anti-CEP55 Recombinant Antibody (clone DS664AB) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY424 | Anti-CEP55 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1381 | Anti-Human CEP55 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC213 | Rabbit Anti-CEP55 Recombinant Antibody (VS13-YC213) | WB, ICC, IF | Rabbit IgG |
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