Rabbit Anti-CEP55 Recombinant Antibody (clone R05-6A6) (CAT#: VS3-FY1986)
This is a rabbit recombinant monoclonal antibody that detects human CEP55. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, ICC/IF. The R05-6A6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200).
Specific Inquiry
WB
Figure 1 Anti-CEP55 Antibody (VS3-FY1986) in WB.
The CEP55 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CEP55 by western blot assay.
ICC
Figure 2 Anti-CEP55 Antibody (VS3-FY1986) in ICC.
Immunocytochemistry staining of CEP55 (green) in Hela using the anti-CEP55 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human CEP55
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CEP55
- Species Reactivity
- Human, Rat
- Clone
- R05-6A6
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.
- Related Disease
- Multinucleated Neurons, Anhydramnios, Renal Dysplasia, Cerebellar Hypoplasia, And Hydranencephaly and Meckel Syndrome, Type 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Centrosomal Protein 55; Up-Regulated In Colon Cancer 6; Cancer/Testis Antigen 111; Centrosomal Protein 55kDa; C10orf3; URCC6
- Gene ID
- 55165
- UniProt ID
- Q53EZ4
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436.
Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary.
Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
- Protein Refseq
- XP_011538220.1; XP_011538221.1; XP_011538222.1; XP_016871861.1; XP_016871862.1
- Function
- Plays a role in mitotic exit and cytokinesis.
Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CEP55"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0664 | Hi-Affi™ Rabbit Anti-CEP55 Recombinant Antibody (clone DS664AB) | WB, ICC, IF, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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