Mouse Anti-ERBB2 Recombinant Antibody (clone 11H9) (CAT#: ZG-0098C)
This product is a mouse antibody that recognizes ERBB2. It can be used in a wide variety of applications, such as: WB, IF/ICC, IHC-P.
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IF
Figure 1 Mouse Anti-ERBB2 Antibody (clone 11H9) in IF
Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HER2 Monoclonal Antibody (11H9) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
WB
Figure 2 Mouse Anti-ERBB2 Antibody (clone 11H9) in WB
WB analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, :reen:primary Antibody was diluted at 1:1000, 4°over night, Secondary antibody was diluted at 1:10000, 37° 1 hour. :ed:Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Secondary antibody was diluted at 1:10000, 37° 1 hour.
IHC
Figure 3 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 4 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 5 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 6 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IF
Figure 7 Mouse Anti-ERBB2 Antibody (clone 11H9) in IF
Immunofluorescence analysis of Rat-spleen tissue. 1, HER2 Monoclonal Antibody (11H9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IHC
Figure 9 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
IHC staining of human breast cancer tissue, diluted at 1:200.
WB
Figure 10 Mouse Anti-ERBB2 Antibody (clone 11H9) in WB
WB analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, (Green) primary Antibody was diluted at 1:1000, 4°over night, Secondary antibody was diluted at 1:10000, 37° 1 hour. (Red) Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Secondary antibody was diluted at 1:10000, 37° 1 hour.
Specifications
- Immunogen
- Synthetic Peptide of HER2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- HER2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 11H9
- Applications
- WB, IF/ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Immunohistochemistry-Paraffin. For details, refer to Tested Data.
Target
- Alternative Names
- Erb-B2 Receptor Tyrosine Kinase 2; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; HER2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog)
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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