Mouse Anti-ERBB2 Recombinant Antibody (clone 11H9) (CAT#: ZG-0098C)
This product is a mouse antibody that recognizes ERBB2. It can be used in a wide variety of applications, such as: WB, IF/ICC, IHC-P.
Specific Inquiry
IF
Figure 1 Mouse Anti-ERBB2 Antibody (clone 11H9) in IF
Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HER2 Monoclonal Antibody (11H9) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
WB
Figure 2 Mouse Anti-ERBB2 Antibody (clone 11H9) in WB
WB analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, :reen:primary Antibody was diluted at 1:1000, 4°over night, Secondary antibody was diluted at 1:10000, 37° 1 hour. :ed:Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Secondary antibody was diluted at 1:10000, 37° 1 hour.
IHC
Figure 3 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 4 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 5 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IHC
Figure 6 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.
IF
Figure 7 Mouse Anti-ERBB2 Antibody (clone 11H9) in IF
Immunofluorescence analysis of Rat-spleen tissue. 1, HER2 Monoclonal Antibody (11H9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IHC
Figure 9 Mouse Anti-ERBB2 Antibody (clone 11H9) in IHC
IHC staining of human breast cancer tissue, diluted at 1:200.
WB
Figure 10 Mouse Anti-ERBB2 Antibody (clone 11H9) in WB
WB analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, (Green) primary Antibody was diluted at 1:1000, 4°over night, Secondary antibody was diluted at 1:10000, 37° 1 hour. (Red) Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, Secondary antibody was diluted at 1:10000, 37° 1 hour.
Specifications
- Immunogen
- Synthetic Peptide of HER2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- HER2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 11H9
- Applications
- WB, IF/ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence, Immunohistochemistry-Paraffin. For details, refer to Tested Data.
Target
- Alternative Names
- Erb-B2 Receptor Tyrosine Kinase 2; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; HER2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog)
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ERBB2"
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-005 | Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) | FC, IP, ELISA, Neut, FuncS, IF, IHC | IgG1 - kappa |
| TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
| TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
| TAB-039CT | Anti-Human HER2/neu Recombinant Antibody (h1E11) | WB | Humanized antibody |
| TAB-041CT | Human Anti-ERBB2 Recombinant Antibody (TAB-041CT) | SPR, ELISA, WB | Humanized antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-066) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-067) | SPR | Llama VHH |
| PNBL-069 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-069) | ELISA | Llama VHH |
| HPAB-1026WJ | Recombinant Camelid Anti-ERBB2 Single Domain Antibody | ELISA | Camelid VHH |
| HPAB-0725-YJ-VHH | Camelid Anti-ERBB2 Recombinant Single Domain Antibody (HPAB-0725-YJ-VHH) | ELISA | Camelid VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-161 | Mouse Anti-ERBB2 Recombinant Antibody (clone chA21); scFv Fragment | FC, WB | Mouse scFv |
| HPAB-0021-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0021-YC-F(E)) | ELISA, Neut, FC | Human Fab |
| FAMAB-0098-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody (clone E402); Fab Fragment | Cyt | Mouse Fab |
| HPAB-0010-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0010-FY-F(E)) | ELISA, FC | Human Fab |
| HPAB-0011-FY-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0011-FY-F(E)) | Inhib | Mouse Fab |
Mouse Antibody
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-042CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (CRX01) | WB | Human antibody |
| TAB-043CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-043CT-S(P)) | ELISA, Inhibion | Human scFv |
| TAB-044CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (MIL5) | WB, FC, IF, Funcs | Human antibody |
| TAB-045CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (Erbicin) | ELISA, Inhibition | Human antibody |
| TAB-048CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-048CT-S(P)) | ELISA, WB | Human scFv |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-014LC | Mouse Anti-ERBB2 Recombinant Antibody (MOB-014LC) | ELISA, FC | Mouse IgG |
| MOB-014LC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (MOB-014LC-F(E)) | ELISA, FC | Mouse Fab |
| MOB-014LC-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (MOB-014LC-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0733LY | Human Anti-ERBB2 Recombinant Antibody (clone AH06) | ELISA | Human IgG |
| HPAB-0734LY | Human Anti-ERBB2 Recombinant Antibody (clone A058) | ELISA | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0641 | PE-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0642 | APC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0643 | FITC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0644 | A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0645 | PE-A*02:01/Human ERBB2 (ILHDGAYSL) MHC Tetramer | FCM, IHC |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP784-YC-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone F/2A); scFv Fragment | ELISA | Mouse scFv |
| HPAB-AP785-YC-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone chF/2A); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1265WJ-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-1265WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-0679YY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone H2-18); scFv Fragment | ELISA | Human scFv |
| FAMAB-0359JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone FRc13); scFv Fragment | DB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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