What type of antibody is this and what is its affinity?

This is a recombinant human monoclonal scFv antibody (clone FRc13) derived from a phage display library. It specifically binds to human ERBB2 with a KD of 20 nM.

What applications can this ERBB2 antibody be used for?

This antibody can be potentially used in breast cancer research. We have validated it for use in Dot Blot (DB), Enzyme-linked Immunosorbent Assay (ELISA), and Functional Studies (FuncS).

Human Anti-ERBB2 Recombinant Antibody (clone FRc13)

CAT#: FAMAB-0359JF

Recombinant monoclonal antibody specially binds to human epidermal growth factor receptor-2 (ERBB2). This human anti-ERBB2 antibody (FRc13) can be potentially used in the breast cancer diagnosis and therapy.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg

Normal Tissue Figure 3 High expression in esophagus

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg

Normal Tissue Figure 5 IHC staining of human urinary bladder

Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg

Normal Tissue Figure 6 Kidney

Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg

Normal Tissue Figure 7 Breast

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2

❮

❯

Specifications

Host Species
Human

Derivation
Human phage display scFv library

Type
Human IgG

Specificity
Human ERBB2

Species Reactivity
Human

Clone
FRc13

Applications
DB, ELISA, FuncS

Related Disease
Breast cancer

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Gene ID
2064

UniProt ID
P04626

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Breast cancer biomarkers at key points during disease progression

High Affinity scFv

This human anti-ERBB2 scFv antibody (clone FRc13) exhibits impressive binding with a KD of 20 nM. We utilized it in ELISA and functional studies. It showed specific binding to human ERBB2, which is critical for our breast cancer research.

Excellent Dot Blot Tool

We used this recombinant antibody for Dot Blot assays to detect ERBB2. The signal was clear and specificity was excellent. Derived from a human phage display library, it offers a unique tool for exploring ERBB2 interactions without animal variability.

Q&As

What type of antibody is this and what is its affinity?

A: This is a recombinant human monoclonal scFv antibody (clone FRc13) derived from a phage display library. It specifically binds to human ERBB2 with a KD of 20 nM.
What applications can this ERBB2 antibody be used for?

A: This antibody can be potentially used in breast cancer research. We have validated it for use in Dot Blot (DB), Enzyme-linked Immunosorbent Assay (ELISA), and Functional Studies (FuncS).

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - FAMAB-0359JF.pdf

MSDS

MSDS - FAMAB-0359JF.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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