Mouse Anti-Histone H3.3 Recombinant Antibody (clone 0725W70)
CAT#: VS-0725-FY237
The Mouse Anti-Histone H3.3 Recombinant Antibody (clone 0725W70) is a recombinant antibody targeting a peptide corresponding to amino acids 21-39 of human Histone H3.3. It is suitable for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) and Western Blot (WB). Histone H3.3 is a histone variant crucial for chromatin structure and gene expression. The SRF (serum response factor) is a transcription factor that binds to the serum response element, highlighting the interplay between histone variants and gene regulation.
Specifications
- Immunogen
- A peptide corresponding to amino acids 21-39 of human Histone H3.3.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Histone H3.3
- Species Reactivity
- Human
- Clone
- 0725W70
- Applications
- Chromatin Immunoprecipitation-Sequencing, Western Blot
- Related Disease
- Diffuse Midline Glioma
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µg per Chromatin Immunoprecipitation
Western Blot: 2 µg/mL
Target
- Alternative Names
- Histone H3.3
- Gene ID
- Long Name
- Histone H3 variant 3 (a specific variant of Histone H3)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Lot Number:
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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