Mouse Anti-Histone H3K27ac Recombinant Antibody (clone 0725W2)
CAT#: VS-0725-FY155
The Mouse Anti-Histone H3K27ac Recombinant Antibody (clone 0725W2) is a recombinant tool for detecting acetyl-lysine 27 on human histone H3. It is generated using a synthetic peptide encompassing this specific modification. This versatile antibody is suitable for a broad range of applications, including ChIP, ChIP-Seq, Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3 is a fundamental component of the nucleosome, which forms the basic unit of chromatin structure, highlighting the antibody's importance in epigenetic research.
Specifications
- Immunogen
- A synthetic peptide that includes acetyl-lysine 27 of human histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H3K27ac
- Species Reactivity
- Human
- Clone
- 0725W2
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Cleavage Under Targets & Release Using Nuclease, Cleavage Under Targets & Tagmentation, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Parkinson's Disease
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Immunocytochemistry/Immunofluorescence: 1-2 µg/mL
Western Blot: 0.5-2 µg/mL
Cleavage Under Targets & Tagmentation: 1 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K27ac
- Long Name
- acetyl-lysine 27 on human histone H3
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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