Mouse Anti-Histone H3K27me3 Recombinant Antibody (clone 0725W8)
CAT#: VS-0725-FY161
The Mouse Anti-Histone H3K27me3 Recombinant Antibody (clone 0725W8) is a recombinant tool designed to detect trimethyl Lys27 of human Histone H3, using a peptide with this specific modification as the immunogen. It is suitable for applications such as Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). This antibody is essential for investigating the role of histone modifications in regulating gene expression and chromatin structure, including critical epigenetic mechanisms.
Specifications
- Immunogen
- A peptide containing trimethyl Lys27 of human Histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H3K27me3
- Species Reactivity
- Human, Mouse
- Clone
- 0725W8
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Diffuse Midline Glioma (including H3K27M mutation)
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 0.5-2 µg/mL
Dot Blot: 0.5-2 µg/mL
Cleavage Under Targets & Tagmentation: 1 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K27me3
- Long Name
- Histone H3 Lysine 27 trimethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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