Mouse Anti-Histone H3K4me1 Recombinant Antibody (clone 0725W4)
CAT#: VS-0725-FY157
The Mouse Anti-Histone H3K4me1 Recombinant Antibody (clone 0725W4) is designed to recognize monomethyl lysine 4 of human histone H3. This recombinant antibody is generated using a synthetic peptide with this specific modification. It is suitable for applications such as Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), and Western Blot (WB). Histone H3 is a fundamental component of the nucleosome, the basic unit of chromatin, making this antibody a valuable resource for studying epigenetic modifications and their impact on chromatin function and gene expression.
Specifications
- Immunogen
- A peptide containing monomethyl lysine 4 of human histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human Histone H3K4me1
- Species Reactivity
- Human
- Clone
- 0725W4
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Cleavage Under Targets & Tagmentation, Dot Blot, Western Blot
- Related Disease
- Colorectal Cancer (CIMP)
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µg each
Chromatin Immunoprecipitation: 10 µL per Chromatin Immunoprecipitation
Western Blot: 1:500-1:2000
Cleavage Under Targets & Tagmentation: 1-2 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K4me1
- Long Name
- Histone H3 Lysine 4 monomethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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