Mouse Anti-Histone H3K4me2 Recombinant Antibody (clone 0725W9)
CAT#: VS-0725-FY162
This recombinant antibody, clone 0725W9, targets dimethyl Lys4 of human histone H3, and was developed using a synthetic peptide featuring this modification. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3 is a core component of the nucleosome, the smallest subunit of chromatin. This antibody is invaluable for exploring the roles of histone modifications in establishing higher-order chromatin structures and influencing gene activity.
Specifications
- Immunogen
- A synthetic peptide that includes dimethyl Lys4 of human histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H3K4me2
- Species Reactivity
- Human, Mouse
- Clone
- 0725W9
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Renal Cancer
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Immunocytochemistry/Immunofluorescence: 1-2 µg/mL
Western Blot: 2-5 µg/mL
Dot Blot: 1 µg/mL
Target
- Alternative Names
- Histone H3K4me2
- Long Name
- Histone H3 Lysine 4 dimethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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