Mouse Anti-Histone H3K4me3 Recombinant Antibody (clone 0725W17)
CAT#: VS-0725-FY173
The Mouse Anti-Histone H3K4me3 Recombinant Antibody (clone 0725W17) is a recombinant antibody designed to detect trimethyl Lys4 of human histone H3, using a synthetic peptide with this modification as its immunogen. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3K4me3 is associated with gene activation, making this antibody a crucial tool for epigenetic research and understanding gene regulation.
Specifications
- Immunogen
- A synthetic peptide that includes trimethyl Lys4 of human histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H3K4me3
- Species Reactivity
- Human
- Clone
- 0725W17
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer, Neurodevelopmental disorders, Cardiovascular diseases
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Western Blot: 2-10 µg/mL
Target
- Alternative Names
- Histone H3K4me3
- Long Name
- Histone H3 Lysine 4 trimethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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