Mouse Anti-Histone H3K9ac Recombinant Antibody (clone 0725W7)
CAT#: VS-0725-FY160
This recombinant antibody, clone 0725W7, targets acetyl-lysine 9 of human Histone H3, with a peptide including this modification serving as the immunogen. It is validated for use in Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3 is a core component of the nucleosome, the fundamental building block of chromatin. This antibody is crucial for studying the impact of histone acetylation and other post-translational modifications on gene expression and chromatin dynamics.
Specifications
- Immunogen
- A peptide that includes acetyl-lysine 9 of human Histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG3
- Specificity
- Human Histone H3K9ac
- Species Reactivity
- Human, Mouse
- Clone
- 0725W7
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Liver Cancer
Product Property
- Purification
- Protein A purified
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 63 mM Trisodium citrate, 70 mM sodium phosphate tribasic, 301 mM sodium chloride, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 2-5 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5 µg each
Western Blot: 0.2-2 µg/mL
Dot Blot: 0.2-2 µg/mL
Cleavage Under Targets & Tagmentation: 1 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K9ac
- Long Name
- Histone H3 Lysine 9 acetylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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