Mouse Anti-Histone H3K9me0 Recombinant Antibody (clone 0725W60)
CAT#: VS-0725-FY227
The Mouse Anti-Histone H3K9me0 Recombinant Antibody (clone 0725W60) is a recombinant antibody targeting a synthetic branched peptide corresponding to the amino acids surrounding Lys9 of human Histone H3. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Enzyme-linked Immunosorbent Assay (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). This antibody is valuable for studying histone modifications and their role in gene expression.
Specifications
- Immunogen
- A synthetic branched peptide corresponding to the amino acids surrounding Lys9 of human Histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Histone H3K9me0
- Species Reactivity
- Human
- Clone
- 0725W60
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Ni-NTA affinity chromatography
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunocytochemistry/Immunofluorescence: 1-2 µg/mL
Western Blot: 0.1-1 µg/mL
Bead-based Enzyme-linked Immunosorbent Assay: 0.5-10 µg/mL
Target
- Alternative Names
- Histone H3K9me0
- Long Name
- Histone H3 Lysine 9 unmethylated (often used to denote the unmodified state)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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