Mouse Anti-Histone H4K16ac Recombinant Antibody (clone 0725W30)
CAT#: VS-0725-FY190
This recombinant antibody, clone 0725W30, targets acetyl-lysine 16 of human Histone H4, using a synthetic peptide with this modification as its immunogen. It is suitable for Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunofluorescence (IF), and Western Blot (WB). Histone H4 is a core component of the nucleosome, which forms the fundamental unit of chromatin. This antibody provides a valuable tool for studying the role of histone acetylation in establishing higher-order chromatin structure and regulating gene expression.
Specifications
- Immunogen
- A synthetic peptide that contains acetyl-lysine 16 of human Histone H4.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H4K16ac
- Species Reactivity
- Human
- Clone
- 0725W30
- Applications
- Chromatin Immunoprecipitation, Dot Blot, Immunofluorescence, Western Blot
- Related Disease
- Cancer, Huntington's disease
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 8 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 2-5 µg per Chromatin Immunoprecipitation
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- Histone H4K16ac
- Long Name
- Histone H4 Lysine 16 acetylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
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Isotype Control
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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