Mouse Anti-Histone H4K20me1 Recombinant Antibody (clone 5E10-D8)
CAT#: VS-0725-FY163
The Mouse Anti-Histone H4K20me1 Recombinant Antibody (clone 5E10-D8) is a recombinant antibody generated against a synthetic peptide containing monomethyl Lys20 of Histone H4. It is designed for applications including Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H4 is a core component of the nucleosome, essential for chromatin structure. This antibody serves as an excellent tool for investigating the role of histone methylation in chromatin organization and gene regulation.
Specifications
- Immunogen
- A synthetic peptide containing monomethyl Lys20 of Histone H4.
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- Human Histone H4K20me1
- Species Reactivity
- Human
- Clone
- 5E10-D8
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Hutchinson-Gilford Progeria Syndrome
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 8 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Immunocytochemistry/Immunofluorescence: 1 µg/mL
Western Blot: 0.5-2 µg/mL
Dot Blot: 1 µg/mL
Target
- Alternative Names
- Histone H4K20me1
- Long Name
- Histone H4 Lysine 20 monomethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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