Mouse Anti-Histone H4K5ac Recombinant Antibody (clone 0725W28)
CAT#: VS-0725-FY188
This recombinant antibody, clone 0725W28, targets acetyl-lysine 5 of human Histone H4, with a synthetic peptide containing this modification as its immunogen. It is validated for Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H4 is a core component of the nucleosome, which is responsible for establishing higher-order chromatin structures. This antibody serves as a valuable tool for studying the role of histone acetylation in chromatin organization and gene regulation.
Specifications
- Immunogen
- A synthetic peptide containing acetyl-lysine 5 of human Histone H4.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Histone H4K5ac
- Species Reactivity
- Human
- Clone
- 0725W28
- Applications
- Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 8 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 2-5 µg per Chromatin Immunoprecipitation
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- Histone H4K5ac
- Long Name
- Histone H4 Lysine 5 acetylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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