Mouse Anti-HSPA9 Recombinant Antibody (clone 3A12G3)
CAT#: VS7-0425-WR96
This product is a mouse IgG1 recombinant antibody that binds to human HSPA9. The antibody 3A12G3 recognizes a specific amino acid domains of human HSPA9 (AA: 480-679). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with monkey and rat.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line A431
Immunofluorescent staining of human cell line A431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9
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Specifications
- Immunogen
- Recombinant human HSPA9 (AA: 480-679) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSPA9
- Species Reactivity
- Human, Monkey, Rat
- Clone
- 3A12G3
- Molecule Class
- Heat Shock Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cancers; Neurodegenerative Disorders; Mitochondrial Diseases
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
- Gene ID
- 3313
- UniProt ID
- P38646
- Long Name
- Heat Shock Protein Family A (Hsp70) Member 9
- Cellular Localization
- Cytoplasm; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_004125.3
- Function
- May regulate the kinase DMPK.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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