Mouse Anti-MFGE8 Recombinant Antibody (clone Mc3) (CAT#: FAMAB-0225CQ)
This product is a recombinant mouse antibody clone Mc3, which specifically binds to BA46.
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Inhib
Figure 1 In vitro adhesion, migration and survival assays to measure effect of MFGE8 on SKOV-3 cells.
Inhibition of adhesion to 5 µg/mL MFGE8 by 10 µg/mL anti-huMFGE8 (hMc3) or a negative control (Rituximab, Ritux).(h) Inhibition of migration to 5 µg/mL huMFGE8 by 10µg/mL anti-huMFGE8 hMc3, as compared to negative control (Ritux). (j) Inhibition of survival in the presence of 10 µg/mL huMFGE8, by 10µg/mL hMc3 or the negative antibody. 100% = basal level of A.U. in the presence of MFGE8. Data are represented as Box-and-whiskers graphs showing the minimum (lower error bar), 25th percentile-median-75th percentile and maximum (upper error bar) values. N=4 (except 3j: N=5). *** = P<0.001; ** = P<0.01; * = P<0.05 compared to Ctrl = PBS in 3b, g; to 0.312 µg/ml MFGE8 in 3i; or to Ctrl = MFGE8 in 3c, d, e, h, j..
Tibaldi, L., Leyman, S., Nicolas, A., Notebaert, S., Dewulf, M., Ngo, T. H., ... & Théry, C. (2013). New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma. PLoS One, 8(8), e72708.
Block
Figure 2 Identification of new antibodies blocking either adhesion, migration or survival induced by MFGE8.
(a) Effect of 10 new anti-MFGE8 antibodies (10 or 50 µg/mL) on adhesion to MFGE8 (adhesion assay), as compared to hMC3. 100% adhesion corresponds to 5 µg/mL MFGE8 coating without antibodies (ctrl). Example of the screening experiments, performed once with each of the 40 new antibodies. Black arrows indicate the antibodies selected for further characterization. (b) Dose–response effect of the 5 selected antibodies, as compared to hMC3 in the adhesion assay. (c) Dose–response effect of the 5 selected antibodies, as compared to hMC3 in the migration assay. 100% migration corresponds to 5 µg/mL MFGE8 without antibodies. (d) Dose–response effect of the 5 selected antibodies, as compared to hMC3 in the survival assay. 100% survival corresponds to 10 µg/mL recombinant MFGE8 without antibodies. Box-and-whiskers graphs as in Figure 3. N=4 (except in (c) for 215A9 -10 µg/mL and 311A7 -0.625 µg/mL: N=3 and for hMc3: N=2; in (b) for 215A9 -5µg/mL: N=2). *** = P<0.001; ** = P<0.01; * = P<0.05 compared to the lowest antibody concentration (0.5 or 0.625 µg/ml) for each individual antibody in 4b, c, d.
Tibaldi, L., Leyman, S., Nicolas, A., Notebaert, S., Dewulf, M., Ngo, T. H., ... & Théry, C. (2013). New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma. PLoS One, 8(8), e72708.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human MFGE8
- Species Reactivity
- Human
- Clone
- Mc3
- Applications
- ELISA, IF, Block, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Blocking.
Target
- Alternative Names
- MFGE8; milk fat globule-EGF factor 8 protein; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
- Gene ID
- 4240
- UniProt ID
- Q08431
Related Resources
Tzioras, Makis, et al. "Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer's disease via MFG-E8." Cell Reports Medicine 4.9 (2023). https://doi.org/10.1016/j.xcrm.2023.101175
This research investigates the role of astrocytes and microglia in Alzheimer's disease (AD) pathology, specifically examining how these glial cells contribute to synapse loss. The study demonstrates that both astrocytes and microglia from human AD brains contain significantly higher amounts of synaptic protein compared to control brains, indicating increased synapse ingestion. This enhanced phagocytosis is exacerbated near amyloid-β plaques and in individuals carrying the APOE4 risk gene. Using in vitro models, the researchers show that mouse and human glial cells preferentially phagocytose synapses derived from AD patients compared to control synapses. Importantly, they identify milk fat globule-EGF factor 8 (MFG-E8) as a key molecular mechanism facilitating this process, showing that blocking MFG-E8 interactions rescues the elevated engulfment of AD synapses without affecting normal synapse uptake.
Creative Biolabs provided the recombinant mouse anti-human MFG-E8 blocking antibody (Mc3, CAT#: FAMAB-0225CQ-LowE) and the mouse IgG1 isotype control antibody (CAT#: MOB-065CQ) for this study. These antibodies were crucial for demonstrating the specific role of MFG-E8 in mediating synaptic phagocytosis, as pre-treatment with the anti-MFG-E8 antibody significantly reduced the ingestion of AD-derived synapses by both astrocytes and microglia. This finding represents a potential therapeutic approach, suggesting that targeting the MFG-E8 pathway could protect synapses from excessive glial engulfment in AD while preserving normal phagocytic functions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-657 | Recombinant Anti-human MFGE8 Antibody | WB, Neut, FuncS | IgG |
| MOB-1240MZ | Recombinant Mouse Anti-Human MFGE8 Antibody (clone FEN56), Biotin-Conjugated | ICC/IF, IHC-P, IP, WB | Mouse antibody |
| HPAB-2103-FY | Human Anti-MFGE8 Recombinant Antibody (clone HuHMFG1) | IA | Human IgG1 |
| HPAB-2104-FY | Mouse Anti-MFGE8 Recombinant Antibody (clone HMFG1) | IA | Mouse IgG |
| ZG-0144U | Mouse Anti-MFGE8 Recombinant Antibody (clone 2B2D10) | ELISA, IHC | Mouse IgG2b |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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