Mouse Anti-PAX6 Recombinant Antibody (clone 1F6G2) (CAT#: VS7-0425-WR567)
This product is a mouse IgG1 antibody that binds to human PAX6. The antibody 1F6G2 recognizes specific amino acid domain of human PAX6 (AA: 1-122). It can be used for IHC and FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/1182_F2_2_red_green.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/1182_F2_2_red_green.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/371_A12_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/371_A12_1_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/372_A12_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/372_A12_1_red_green.jpg
Figure 4 RNA cell line category: Cell line enhanced (AF22, AN3-CA, HEK93, HeLa, hTERT-RPE1, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007372-PAX6
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007372-PAX6
Specifications
- Immunogen
- Recombinant human PAX6 (AA: 1-122) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAX6
- Species Reactivity
- Human
- Clone
- 1F6G2
- Molecule Class
- Transcription Factor
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Aniridia 1 (AN1); Anterior segment dysgenesis 5 (ASGD5); Foveal hypoplasia 1 (FVH1); Keratitis hereditary (KERH); Microphthalmia/coloboma 12 (MCOPCB12); Coloboma of optic nerve (COLON); Bilateral optic nerve hypoplasia (BONH); Aniridia 2 (AN2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PAX6; paired box 6; AN; AN2; FVH1; MGDA; WAGR; D11S812E; paired box protein Pax-6; aniridia type II protein; oculorhombin; paired box homeotic gene-6
- Gene ID
- 5080
- UniProt ID
- P26367
- Long Name
- Paired Box 6
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubl conjugation
- Protein Refseq
- NP_000271.1
- Function
- Developmental protein; DNA-binding; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2115MZ | Recombinant Mouse Anti-Human PAX6 Antibody (clone 2D9) | ELISA, ICC/IF, WB | Mouse antibody |
| MRO-1171-CN | Recombinant Rabbit Anti-PAX6 Monoclonal Antibody (CBACN-428) | WB, IHC, FC | Rabbit IgG |
| MRO-1172-CN | Recombinant Rabbit Anti-PAX6 Monoclonal Antibody (CBACN-429) | WB, IF, IHC | Rabbit IgG |
| VS3-XY1214 | Mouse Anti-PAX6 Recombinant Antibody (clone 1C8) | ELISA, WB, FC | Mouse IgG1 |
| VS3-FY1098 | Recombinant Rabbit Anti-PAX6 Antibody (clone R01-1G8) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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