Rabbit Anti-PAX6 Recombinant Antibody (clone R03-8G4)
CAT#: VS3-FY1815
This anti-PAX6 antibody can be used in western blot analysis of PAX6 in rat brain lysates. The recombinant monoclonal antibody specifically reacts with human and rat PAX6. The R03-8G4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/1182_F2_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/371_A12_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30775/372_A12_1_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, AN3-CA, HEK93, HeLa, hTERT-RPE1, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007372-PAX6
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WB
Figure 1 Anti-PAX6 Antibody (VS3-FY1815) in WB.
The PAX6 expression in rat Brain lysates were detected using the anti-PAX6 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptides of human PAX6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PAX6
- Species Reactivity
- Human, Rat
- Clone
- R03-8G4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 47 kDa.
- Related Disease
- Coloboma Of Optic Nerve and Coloboma, Ocular, Autosomal Dominant
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Paired Box 6; Aniridia Type II Protein; Oculorhombin; AN2; Paired Box Gene 6 (Aniridia, Keratitis); Paired Box Homeotic Gene-6; Paired Box Protein Pax-6; Aniridia, Keratitis
- Gene ID
- 5080
- UniProt ID
- P26367
- Sequence Similarities
- Belongs to the paired homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
- Protein Refseq
- NP_000271.1; NP_001121084.1; NP_001245393.1
- Function
- Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity).
Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity).
Acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PAX6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2602 | Hi-Affi™ Recombinant Rabbit Anti-PAX6 Monoclonal Antibody (DS2602AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1171-CN | Recombinant Rabbit Anti-PAX6 Monoclonal Antibody (CBACN-428) | WB, IHC, FC | Rabbit IgG |
| VS3-XY1214 | Mouse Anti-PAX6 Recombinant Antibody (clone 1C8) | ELISA, WB, FC | Mouse IgG1 |
| VS3-FY1098 | Recombinant Rabbit Anti-PAX6 Antibody (clone R01-1G8) | WB | Rabbit IgG |
| VS7-0425-WR567 | Mouse Anti-PAX6 Recombinant Antibody (clone 1F6G2) | IHC, FC | Mouse IgG1 |
| VS7-0425-WR568 | Mouse Anti-PAX6 Monoclonal Antibody (VS7-0425-WR568) | WB, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1570 | Anti-PAX6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5207 | Anti-Mouse PAX6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5206 | Anti-Human PAX6 Immunohistochemistry Kit | IHC |
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