Rabbit Anti-SPTAN1 Recombinant Antibody (clone R01-9G3)
CAT#: VS3-XY3513
The rabbit recombinant antibody specifically recognizes SPTAN1 (Spectrin alpha, non-erythrocytic 1). This antibody specifically reacts with human, mouse and rat SPTAN1. It has been tested using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to microtubules & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7927/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_7_3.jpg
Normal Tissue
Figure 4 Pancreas
Exocrine glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_1_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4581/13221_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197694-SPTAN1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SPTAN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-9G3
- Applications
- WB, IHC-P, IP
- Related Disease
- Developmental And Epileptic Encephalopathy 5; Neuronopathy, Distal Hereditary Motor, Autosomal Dominant 11
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- DEE5; NEAS; DEVEP; EIEE5; HMN11; SPG91; SPTA2; HMND11
- Gene ID
- 6709
- UniProt ID
- Q13813
- Long Name
- Spectrin alpha, non-erythrocytic 1
- Cellular Localization
- Cytoplasm, cytoskeleton, cell cortex
- Post Translation Modifications
- Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. (Q13813-SPTN1_HUMAN)
Ubiquitination at Lys1519 and Lys2426 (NX_Q13813 [NX_Q13813-1])
Modification sites at PhosphoSitePlus (Q13813)
Glycosylation from GlyGen (Q13813) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016870549.1
- Function
- Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SPTAN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1304z | Mouse Anti-SPTAN1 Recombinant Antibody (clone 32D6) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-0596MZ | Recombinant Mouse Anti-Human SPTAN1 Antibody (clone E9C8) | ELISA, IHC-Fr, IHC-P, IP, WB | Mouse antibody |
| MRO-0066-CN | Recombinant Rabbit Anti-SPTAN1 Monoclonal Antibody (JU32-09) | WB, IHC | Rabbit IgG |
| VS3-FY1385 | Recombinant Rabbit Anti-SPTAN1 Antibody (clone R08-7C6) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3400 | Hi-Affi™ Recombinant Rabbit Anti-SPTAN1 Monoclonal Antibody (DS3400AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2165 | Anti-SPTAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6879 | Anti-Mouse SPTAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6880 | Anti-Rat SPTAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6878 | Anti-Human SPTAN1 Immunohistochemistry Kit | IHC |
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